Bishara A, Malka R, Brautbar C, Barak V, Cohen I, Kedar E
Tissue Typing Unit, Hadassah University Hospital, Jerusalem, Israel.
J Immunol Methods. 1998 Jun 1;215(1-2):187-90. doi: 10.1016/s0022-1759(98)00078-7.
The mixed leukocyte reaction (MLR) is an in vitro test commonly performed in a serum-containing medium (SCM), and used to study allorecognition and cellular immunity accompanied by cytokine release. We investigated the possibility of performing the MLR test in serum-free media (SFM) by comparing human leukocyte proliferation and cytokine release in MLRs performed in SFM and SCM. Of the four SFM tested, only Biotarget- was as effective as SCM in supporting leukocyte proliferation and IL-2 secretion. Both phenomena were observed only in allogeneic combinations. The levels of IL-1, IL-6, and TNFalpha in allogeneic MLR combinations in SFM were half those in SCM cultures; the kinetics of their release were the same. With the exception of IL-2, a high degree of spontaneous release of the other three cytokines analyzed was observed in responder cells, in irradiated stimulator cells, and in autologous combinations cultured in both SCM and SFM. It appears that unlike IL-2, the cytokines IL-1, IL-6, and TNFalpha are nonspecifically produced in MLR and cannot serve as sensitive indices of HLA disparity.
混合淋巴细胞反应（MLR）是一种通常在含血清培养基（SCM）中进行的体外试验，用于研究同种异体识别和伴随细胞因子释放的细胞免疫。我们通过比较在无血清培养基（SFM）和SCM中进行的MLR中人类白细胞增殖和细胞因子释放情况，研究了在无血清培养基中进行MLR试验的可能性。在所测试的四种SFM中，只有Biotarget-在支持白细胞增殖和IL-2分泌方面与SCM一样有效。这两种现象仅在同种异体组合中观察到。SFM中同种异体MLR组合中IL-1、IL-6和TNFα的水平是SCM培养物中的一半；它们的释放动力学相同。除IL-2外，在应答细胞、经辐照的刺激细胞以及在SCM和SFM中培养的自体组合中，观察到所分析的其他三种细胞因子有高度的自发释放。似乎与IL-2不同，细胞因子IL-1、IL-6和TNFα在MLR中是非特异性产生的，不能作为HLA差异的敏感指标。
