Potashkin J, Kim D, Fons M, Humphrey T, Frendewey D
Department of Cellular and Molecular Pharmacology, Finch University of Health Sciences, The Chicago Medical School, North Chicago, IL 60064, USA.
Curr Genet. 1998 Sep;34(3):153-63. doi: 10.1007/s002940050381.
We have isolated six new pre-mRNA splicing mutants (prp) from a collection of temperature-sensitive (ts-) Schizosaccharomyces pombe strains. The prp mutants are defective in the splicing of both messenger RNA and U6 small nuclear RNA precursors. A single recessive mutation is responsible for both the ts- growth and the splicing phenotypes in each of the prp mutants. The six prp mutations are unlinked and fall into separate complementation groups. Two are allelic with the previously described prp3 and prp4 mutations; the remaining four define the new alleles prp5-1, prp6-1, prp7-1, and prp9-1. The six mutants exhibit three splicing phenotypes: accumulation of unspliced precursor at the restrictive but not at the permissive temperature; accumulation of unspliced precursor at both the permissive and restrictive temperatures; and accumulation of unspliced precursor, the intron-exon lariat intermediate, and the intron lariat final product. In addition to their aberrant splicing phenotypes, the prp5-1 and prp6-1 mutants express classical cell-division-cycle defects, while prp7-1 exhibits an unusual hyphal morphology. These results suggest a connection between pre-mRNA splicing and the control of cell division in fission yeast.
我们从一组温度敏感型（ts-）粟酒裂殖酵母菌株中分离出了六个新的前体mRNA剪接突变体（prp）。prp突变体在信使RNA和U6小核RNA前体的剪接过程中存在缺陷。每个prp突变体中，单个隐性突变导致了ts-生长表型和剪接表型。这六个prp突变不连锁，分属于不同的互补群。其中两个与先前描述的prp3和prp4突变等位；其余四个定义了新的等位基因prp5-1、prp6-1、prp7-1和prp9-1。这六个突变体表现出三种剪接表型：在限制温度而非允许温度下积累未剪接的前体；在允许温度和限制温度下均积累未剪接的前体；以及积累未剪接的前体、内含子-外显子套索中间体和内含子套索终产物。除了异常的剪接表型外，prp5-1和prp6-1突变体还表现出典型的细胞分裂周期缺陷，而prp7-1表现出异常的菌丝形态。这些结果表明，前体mRNA剪接与裂殖酵母中的细胞分裂控制之间存在联系。
