一种猕猴抗Rh17样单克隆抗体的特性鉴定
Characterization of a macaque anti-Rh17-like monoclonal antibody.
作者信息
Blancher A, Roubinet F, Reid M E, Socha W W, Bailly P, Bénard P
机构信息
Laboratoire d'Immunogénétique Moléculaire, Université Paul-Sabatier, Hôpital Purpan, Toulouse, France.
出版信息
Vox Sang. 1998;75(1):58-62.
In order to produce macaque monoclonal antibodies (mAbs) against human red blood cell (RBC) antigens, macaques were immunized with human and gorilla RBCs and their spleen lymphocytes were fused with man-mouse heteromyeloma cells. One macaque-mouse heterohybridoma produced a macaque IgGx (Cvn2-4D5) which agglutinated all human RBCs but not rare human variants Dc-,D-, and Rhnull. Thus, Cyn2-4D5 exhibited RH17-like reactivity. The specificity of Cyn2-4D5 for RHCE-encoded polypeptides was confirmed by specific immunoprecipitation of RhcE and RhCe polypeptides from human RBCs and the absence of immunoprecipitation of the RhD polypeptides extracted from D-RBCs. This study demonstrates that it is possible to produce macaque mAbs against human RBC blood group antigens.
为了产生针对人类红细胞（RBC）抗原的猕猴单克隆抗体（mAb），用人类和大猩猩红细胞免疫猕猴，并将其脾脏淋巴细胞与人鼠异源骨髓瘤细胞融合。一种猕猴 - 小鼠异源杂交瘤产生了一种猕猴IgGx（Cvn2 - 4D5），它能凝集所有人类红细胞，但不能凝集罕见的人类变体Dc-、D-和Rhnull。因此，Cyn2 - 4D5表现出类似RH17的反应性。通过从人类红细胞中特异性免疫沉淀RhcE和RhCe多肽以及未从D型红细胞中提取的RhD多肽进行免疫沉淀，证实了Cyn2 - 4D5对RHCE编码多肽的特异性。这项研究表明，有可能产生针对人类红细胞血型抗原的猕猴单克隆抗体。
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