Ou Y C, Chen J T, Yang C R, Horng Y Y, Kao Y L, Cheng C L
Department of Surgery, Taichung Veterans General Hospital, Taiwan, ROC.
Zhonghua Yi Xue Za Zhi (Taipei). 1998 Aug;61(8):441-7.
Experience suggests that tumor growth is dependent on angiogenesis. The intensity of angiogenesis in human cancer is reported to be predictive of the probability of metastasis in many types of cancer. The aims of this study were 1) to determine the relationship of microvessel density (MVD) in renal cell carcinoma to pathologic stage, and 2) to evaluate the role of MVD in metastasis.
Paraffin-embedded tumor specimens were reviewed from 34 unselected patients with RCC who had undergone surgery from 1986 to 1990 at Taichung Veterans General Hospital. The pathology findings and clinical records were reviewed to note relationships between pathologic stage and whether or not metastasis had occurred. Specimens were studied from 16 cases (eight Stage I cancers, five Stage II and three Stage III) without metastasis and from 18 cases (two Stage I, six Stage II, six Stage III and four Stage IV) in which metastasis later developed. Microvessels were highlighted by immunostaining endothelial cells for factor VIII-related antigen. Microvessels were counted in a x-400 field (0.1885 mm2/field) in the most active areas of neovascularization.
The 16 patients without metastasis have survived for between 65 and 136 months (mean, 94.5 months), up to the present time. Of the 18 patients with metastasis, 15 died and three survived, with mean survivals of 42.8 months (range, 12-99 months). Mean overall MVD was 99.6 vessels; mean MVD was 98.5, 96.2, 109.3 and 90.0 in Stages I, II, III and IV tumors, respectively. Mean MVD was 99.3 in patients without metastasis and 99.9 in patients with metastasis.
MVD does not correlate with pathologic stage and is of no prognostic significance in renal cell carcinoma.
经验表明肿瘤生长依赖于血管生成。据报道，人类癌症中血管生成的强度可预测多种类型癌症发生转移的可能性。本研究的目的是：1）确定肾细胞癌中微血管密度（MVD）与病理分期的关系，以及2）评估MVD在转移中的作用。
回顾了1986年至1990年在台中荣民总医院接受手术的34例未经选择的肾细胞癌患者的石蜡包埋肿瘤标本。审查病理结果和临床记录，以记录病理分期与是否发生转移之间的关系。研究了16例无转移病例（8例I期癌症、5例II期和3例III期）以及18例后来发生转移的病例（2例I期、6例II期、6例III期和4例IV期）的标本。通过对内皮细胞进行因子VIII相关抗原免疫染色来突出微血管。在新生血管最活跃的区域，在x400视野（0.1885平方毫米/视野）中计数微血管。
16例无转移患者至今已存活65至136个月（平均94.5个月）。18例发生转移的患者中，15例死亡，3例存活，平均生存期为42.8个月（范围12 - 99个月）。总体平均MVD为99.6个血管；I、II、III和IV期肿瘤的平均MVD分别为98.5、96.2、109.3和90.0。无转移患者的平均MVD为99.3，发生转移患者的平均MVD为99.9。
MVD与肾细胞癌的病理分期无关，对肾细胞癌无预后意义。
