Dumitriu S, Băncescu G, Murea A, Skaug N
University of Medicine and Pharmacy Carol Davila, Bucharest, Romania.
Roum Arch Microbiol Immunol. 1998 Jan-Mar;57(1):5-10.
The aims of the study were to isolate and to identify at species level the Prevotella strains in pus samples collected by needle aspiration from 25 Romanian patients with periodontal abscesses. Gram-stained smears and cultures on selective and nonselective media were performed from each of the 25 pus samples. The isolates were identified on the basis of Gram staining, cultural characteristics and standard biochemical reactions. The Gram-negative anaerobic bacilli isolates were biochemically characterized and identified at species level using the Rapid ID 32 A system (Bio Mérieux, France). Fifteen Prevotella isolates belonging to one of the following species: P. melaninogenica, P. denticola, P. oralis, P. loescheii and P. bivia were recovered. All Prevotella isolates reacted similarly in 20 tests in the Rapid ID 32 A system. The P. melaninogenica strain showed approximately the same biochemical profile and only two sugar fermentation tests were not constantly positive. The study confirmed that Prevotella is often involved in periodontal abscesses (> 50% of the cases) in association with other anaerobic or/and aerobic bacteria. P. melaninogenica was the most frequently isolated Prevotella species from the investigated cases.
本研究的目的是从25例罗马尼亚牙周脓肿患者经针吸采集的脓液样本中分离出普雷沃菌属菌株，并在种水平上进行鉴定。对25份脓液样本分别进行革兰氏染色涂片以及在选择性和非选择性培养基上培养。根据革兰氏染色、培养特性和标准生化反应对分离菌株进行鉴定。使用Rapid ID 32 A系统（法国生物梅里埃公司）对革兰氏阴性厌氧杆菌分离株进行生化特性分析并在种水平上进行鉴定。共分离出15株普雷沃菌属菌株，分别属于以下物种之一：产黑色素普雷沃菌、栖牙普雷沃菌、口腔普雷沃菌、洛氏普雷沃菌和二路普雷沃菌。所有普雷沃菌属分离株在Rapid ID 32 A系统的20项检测中反应相似。产黑色素普雷沃菌菌株表现出大致相同的生化特征，只有两项糖发酵试验并非始终呈阳性。该研究证实，普雷沃菌属常与其他厌氧或/需氧菌一起参与牙周脓肿（>50%的病例）。产黑色素普雷沃菌是在所调查病例中最常分离出的普雷沃菌属物种。
