Dolganiuc A, Radu D, Olinescu A, Vrăbiescu A
Cantacuzino Institute, Bucharest, Romania.
Roum Arch Microbiol Immunol. 1998 Jan-Mar;57(1):23-32.
The investigations were conducted on 3 groups of New Zealand rabbits: 1) controls; 2) injected with procain, i.m. 15 mg/kg body weight, daily, for 30 days; 3) injected with diethylaminoethanol (DEAE), 15 mg/kg body weight, daily, for 35 days. The study was made also on human leukocytes, isolated from the peripheral blood of 10 clinically healthy subjects (adults), procain and DEAE action being investigated in vitro. The free oxygen radicals (FOR) released by PMN leukocytes were evaluated by chemiluminescence, in vitro. Addition of procain or DEAE had no effect on the release of FOR by PMN leukocytes of control rabbits. In the experiment made on rabbits treated with procain or DEAE, the release of FOR by PMN leukocytes was much more reduced, as compared to controls. In the rabbits treated with procain, the intensity of the emitted light was 2.27 mV, in those treated with DEAE, 3.46 mV, while in the controls, the mean value was 6.74 mV. In the in vitro experiments performed on human PMN cells stimulated with opsonized zymosan (OZ), addition of procain or DEAE had an inhibiting effect on the FOR release. As compared to control, the means of the FOR values decreased from 59 to 41.2 mV in case of procain addition and from 67.7 to 50 mV in case of DEAE addition. The fact that the inflammation is associated with accumulation of free radicals, suggests the opportunity to test these substances, especially DEAE, as antioxidant agents.
1）对照组；2）每天肌肉注射15mg/kg体重的普鲁卡因，持续30天；3）每天肌肉注射15mg/kg体重的二乙氨基乙醇（DEAE），持续35天。还对从10名临床健康受试者（成年人）外周血中分离出的人类白细胞进行了研究，在体外研究了普鲁卡因和DEAE的作用。通过化学发光法在体外评估PMN白细胞释放的游离氧自由基（FOR）。添加普鲁卡因或DEAE对对照兔PMN白细胞释放FOR没有影响。在用普鲁卡因或DEAE处理的兔子所做的实验中，与对照组相比，PMN白细胞释放FOR的量减少得更多。在用普鲁卡因处理的兔子中，发光强度为2.27mV，在用DEAE处理的兔子中为3.46mV，而在对照组中，平均值为6.74mV。在用调理酵母聚糖（OZ）刺激的人类PMN细胞上进行的体外实验中，添加普鲁卡因或DEAE对FOR释放有抑制作用。与对照相比，添加普鲁卡因时FOR值的平均值从59降至41.2mV，添加DEAE时从67.7降至50mV。炎症与自由基积累相关这一事实表明，有机会测试这些物质，尤其是DEAE，作为抗氧化剂。
