HeLa细胞中细胞周期蛋白B1在辐射诱导的G2/M期阻滞消除后的表达情况。
Cyclin B1 expression in response to abrogation of the radiation-induced G2/M block in HeLa cells.
作者信息
Theron T, Böhm L
机构信息
Department of Radiation Oncology, University of Stellenbosch Medical Faculty, Tygerberg, South Africa.
出版信息
Cell Prolif. 1998 Apr;31(2):49-57. doi: 10.1046/j.1365-2184.1998.00107.x.
The G2 block is a major response of cells to DNA damage and seem to be induced independently of p53 status. It is thought that the G2 block has a protective function and allows cells to repair their DNA. The molecular events involved in the formation of the G2 block therefore are of great interest. We have used pentoxifylline, a potent G2 delay abrogator, to study the expression of an essential component of the mitosis promoting complex (MPF), cyclin B1. Cyclin B1/G2 ratios are used to show that irradiation induces a decrease in cyclin B1 expression and that pentoxifylline restores cyclin B1 expression to control level. This confirms that suppression of cyclin B1 plays a role in the formation of the G2 cell cycle delay, and that elevating cyclin B1 expression is part of the mechanism of action of pentoxifylline on G2 blocked cells.
G2期阻滞是细胞对DNA损伤的主要反应，且似乎独立于p53状态而被诱导。据认为，G2期阻滞具有保护功能，能使细胞修复其DNA。因此，参与G2期阻滞形成的分子事件备受关注。我们使用了己酮可可碱，一种有效的G2期延迟消除剂，来研究有丝分裂促进复合物（MPF）的一个重要成分细胞周期蛋白B1的表达。细胞周期蛋白B1与G2期的比率用于表明辐射诱导细胞周期蛋白B1表达下降，且己酮可可碱将细胞周期蛋白B1表达恢复到对照水平。这证实了细胞周期蛋白B1的抑制在G2期细胞周期延迟的形成中起作用，且提高细胞周期蛋白B1的表达是己酮可可碱对G2期阻滞细胞作用机制的一部分。
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