Konfortov B A, Jørgensen C B, Miller J R, Tucker E M
Department of Immunology, Babraham Institute, Cambridge, UK.
Anim Genet. 1998 Aug;29(4):302-6. doi: 10.1046/j.1365-2052.1998.00336.x.
A bovine/murine hybrid cell panel consisting of 57 cell lines was typed with 124 markers by PCR. Southern hybridisation and isozyme analysis in order to establish its utility as a resource for genome mapping. All bovine chromosomes, including the sex chromosomes were represented in the panel. Computerised analysis of syntenies indicated that there are no cell lines containing only a single bovine chromosome. The panel was used to map 10 new bovine microsatellite markers, and the MYL6 and CPE genes. This panel is informative for all bovine chromosomes other than the sex-specific region of the X chromosome and can be used in synteny mapping studies. At present, due to the relatively small number of markers typed, the resolution of the panel does not go beyond the chromosomal level.
由57个细胞系组成的牛/鼠杂交细胞板通过聚合酶链反应（PCR）用124个标记进行分型。采用Southern杂交和同工酶分析，以确定其作为基因组作图资源的效用。该细胞板包含了所有牛染色体，包括性染色体。对同线性的计算机分析表明，没有仅包含一条牛染色体的细胞系。该细胞板用于定位10个新的牛微卫星标记以及MYL6和CPE基因。除X染色体的性别特异性区域外，该细胞板对所有牛染色体都具有信息价值，可用于同线性作图研究。目前，由于分型标记数量相对较少，该细胞板的分辨率尚未超出染色体水平。
