Stefanović V, Golubović E, Mitić-Zlatković M, Vlahović P, Jovanović O, Bogdanović R
Institute of Nephrology and Hemodialysis, Faculty of Medicine, Nis, Yugoslavia.
Pediatr Nephrol. 1998 Aug;12(6):463-6. doi: 10.1007/s004670050488.
Cellular immune disturbances, and T lymphocyte function in particular, have been previously implicated in idiopathic nephrotic syndrome (INS) of childhood. There are different patterns of cytokine expression in various forms of glomerulonephritis, which suggests that local production of these peptides plays an important role in the pathogenesis and progression of glomerulonephritis. To investigate T-cell and monocyte/macrophage cytokine production in INS, interleukin-12 (IL-12) and interferon-gamma (IFN-gamma) production by peripheral blood mononuclear cells (PBMC) of 11 children with steroid-sensitive nephrotic syndrome (SSNS), 9 with focal segmental glomerulosclerosis (FSGS), and 17 healthy controls was determined. Children with SSNS were studied in relapse, during corticosteroid treatment, and in stable remission, off corticosteroid treatment. IL-12 was not detected in serum, urine, and in supernatants of unstimulated PBMC. IL-12 production by concanavalin A (Con A)-stimulated PBMC of children with SSNS and FSGS was not different from controls. IFN-gamma production by Con A-stimulated PBMC was decreased in children with relapsing SSNS, both in relapse and and during corticosteroid treatment. However, in stable remission it was similar to controls. Markedly decreased IFN-gamma production (P<0.001) was observed by pokeweed mitogen-stimulated PBMC of relapsing SSNS patients and moderately decreased production by PBMC of FSGS patients. This study has established a decreased production of IFN-gamma by PBMC of relapsing SSNS and FSGS patients, but does not allow differentiation between these two different conditions. IL-12 did not have a pathogenic role in either SSNS or FSGS.
细胞免疫紊乱，尤其是T淋巴细胞功能，先前已被认为与儿童特发性肾病综合征（INS）有关。在各种形式的肾小球肾炎中存在不同的细胞因子表达模式，这表明这些肽的局部产生在肾小球肾炎的发病机制和进展中起重要作用。为了研究INS中T细胞和单核细胞/巨噬细胞的细胞因子产生情况，测定了11例激素敏感型肾病综合征（SSNS）患儿、9例局灶节段性肾小球硬化症（FSGS）患儿和17名健康对照者外周血单个核细胞（PBMC）产生白细胞介素-12（IL-12）和干扰素-γ（IFN-γ）的情况。对SSNS患儿在复发期、糖皮质激素治疗期间以及停用糖皮质激素处于稳定缓解期进行了研究。在血清、尿液和未刺激的PBMC上清液中未检测到IL-12。SSNS和FSGS患儿经刀豆蛋白A（Con A）刺激的PBMC产生IL-12的情况与对照组无差异。复发期SSNS患儿经Con A刺激的PBMC产生IFN-γ的能力在复发期和糖皮质激素治疗期间均降低。然而，在稳定缓解期，其与对照组相似。复发期SSNS患者经商陆有丝分裂原刺激的PBMC观察到IFN-γ产生明显降低（P<0.001），FSGS患者的PBMC产生IFN-γ则中度降低。本研究证实复发期SSNS和FSGS患者的PBMC产生IFN-γ减少，但无法区分这两种不同情况。IL-12在SSNS或FSGS中均无致病作用。
