Doyle J M, McCord B R
Forensic Science Research and Training Center, FBI Academy, Quantico, VA 22135, USA.
J Chromatogr B Biomed Sci Appl. 1998 Aug 28;714(1):105-11. doi: 10.1016/s0378-4347(98)00248-5.
A novel electrolyte has been developed for the effective separation by capillary electrophoresis of cations detected in low explosive residue. This electrolyte, with a pH of 4.4, employs 17.5 mM alpha-hydroxyisobutyric acid (HIBA) as the complexing agent, 6 mM imidazole as the ultraviolet visualization agent, 4 mM 18-crown-6 ether as a modifier to enhance the selectivity of the inorganic cations, and 5% (v/v) acetonitrile as an organic additive. Studies which assessed the value of the addition of 18-crown-6 and acetonitrile demonstrated conclusively that both were required in order to achieve unambiguous baseline separation of ammonium, potassium and monomethylammonium ions. The major advantages of the use of this electrolyte are a total run time of less than 7 min and symmetrical peak shapes. Validation on a series of preblast and postblast explosives materials determined that this procedure is reliable and robust.
一种新型电解质已被开发出来，用于通过毛细管电泳有效分离低爆炸物残留物中检测到的阳离子。这种pH值为4.4的电解质采用17.5 mM的α-羟基异丁酸（HIBA）作为络合剂，6 mM咪唑作为紫外可视化剂，4 mM 18-冠-6醚作为改性剂以提高无机阳离子的选择性，以及5%（v/v）乙腈作为有机添加剂。评估添加18-冠-6和乙腈价值的研究最终表明，为了实现铵离子、钾离子和一甲铵离子的明确基线分离，两者都是必需的。使用这种电解质的主要优点是总运行时间少于7分钟且峰形对称。对一系列爆炸前和爆炸后的爆炸物材料进行的验证确定该程序可靠且稳健。
