Plomann M, Lange R, Vopper G, Cremer H, Heinlein U A, Scheff S, Baldwin S A, Leitges M, Cramer M, Paulsson M, Barthels D
Institut für Biochemie II, Med. Fakultät, Universität zu Köln, Germany.
Eur J Biochem. 1998 Aug 15;256(1):201-11. doi: 10.1046/j.1432-1327.1998.2560201.x.
To identify genes that are differentially expressed during self-repair processes in mouse brain, we screened a subtracted cDNA library enriched for brain-specific clones. One of these clones, H74, detected a 4.4-kb mRNA predominantly expressed in brain and dorsal root ganglia neurons. Expression increased continuously during the lifespan and the state of differentiation, but decreased after entorhinal-cortex lesion. A full-length cDNA clone was isolated from a cerebellum cDNA library and characterized. Sequence analysis and database search revealed high sequence similarity to FAP52, a protein expressed in focal-adhesion contacts, and uncharacterized Echinococcus and Caenorhabditis elegans gene products. Furthermore, peptide sequences derived from human cDNA fragments showed up to 65% sequence identity at the amino acid level. The presence of a C-terminal src homology 3 (SH3) domain and its phosphorylation by casein kinase 2 (CK2) and protein kinase C (PKC) imply a role in signaling. Here we demonstrate that the gene encodes a phosphoprotein, referred to as PACSIN, with a restricted spatial and temporal expression pattern.
为了鉴定在小鼠大脑自我修复过程中差异表达的基因，我们筛选了一个富含脑特异性克隆的消减cDNA文库。其中一个克隆H74检测到一个4.4kb的mRNA，主要在大脑和背根神经节神经元中表达。在整个生命周期和分化状态中表达持续增加，但在内嗅皮质损伤后表达下降。从一个小脑cDNA文库中分离出一个全长cDNA克隆并进行了表征。序列分析和数据库搜索显示，它与FAP52具有高度的序列相似性，FAP52是一种在粘着斑接触中表达的蛋白质，此外还与未鉴定的细粒棘球绦虫和秀丽隐杆线虫基因产物相似。此外，源自人类cDNA片段的肽序列在氨基酸水平上显示出高达65%的序列同一性。其C末端src同源3（SH3）结构域的存在以及酪蛋白激酶2（CK2）和蛋白激酶C（PKC）对其的磷酸化表明它在信号传导中起作用。在这里，我们证明该基因编码一种磷蛋白，称为PACSIN，具有受限的时空表达模式。
