Herrlinger K, Eckstein V, Westphal E, Hamelmann W, Thiede A, Ulrichs K
Chirurgische Klinik und Poliklinik, Universität Würzburg.
Zentralbl Chir. 1998;123(7):803-8.
The worldwide lack of human organ donors puts the pig as potential xenogeneic donor species into the prime of interest. Aim of the present in vitro study is the analysis of T-cell activation in the clinically attractive combination "pig-to-human". Peripheral human blood leukocytes (hPBL) and peripheral porcine blood leukocytes (pPBL) were co-cultured for 4-8 days in the xenogeneic mixed lymphocyte reaction (xMLR) and cell proliferation was measured by 3H-thymidine uptake. Both cell populations were separated into T-cells and antigen presenting cells (APC) to analyze direct and indirect antigen recognition. The results show that (a) activation of human T-cells occurs, (b) the strength of activation depends e.g. on the human responder ("high" and "low" responders), (c) the strength of activation is independent of the responder's HLA-DR status, and (d) direct T-cell activation dominates over indirect activation. Thus, T-cell activation is another immunological barrier that has to be overcome before xenotransplantation can be clinically approached.
全球人体器官捐献者的短缺使猪作为潜在的异种供体物种备受关注。本项体外研究的目的是分析临床上有吸引力的“猪对人”组合中的T细胞活化情况。在异种混合淋巴细胞反应（xMLR）中，将人外周血白细胞（hPBL）和猪外周血白细胞（pPBL）共培养4至8天，并通过3H-胸腺嘧啶核苷摄取来测量细胞增殖。将这两种细胞群体分离为T细胞和抗原呈递细胞（APC），以分析直接和间接抗原识别。结果表明：（a）人T细胞发生活化；（b）活化强度例如取决于人类应答者（“高”和“低”应答者）；（c）活化强度与应答者的HLA-DR状态无关；（d）直接T细胞活化比间接活化占主导。因此，T细胞活化是异种移植临床应用之前必须克服的另一个免疫障碍。
