Davido D J, Leib D A
Department of Ophthalmology and Visual Sciences, Washington University School of Medicine, St Louis, MO 63110, USA.
J Gen Virol. 1998 Sep;79 ( Pt 9):2093-8. doi: 10.1099/0022-1317-79-9-2093.
Sequences from -420 to -70 from the ICPO transcriptional start site of herpes simplex virus type 1 are dispensable for reactivation from latency. A putative cAMP-response element (CRE) outside of this region was non-functional in both murine neuroblastoma (NB41A3) and rat pheochromocytoma (PC12) cells. Also, poor binding of cAMP-response element binding protein (CREB) was observed. Sequences from -95 to -37 are important for constitutive activity in NB41A3, PC12 and baby hamster kidney (BHK) cells. The TATA box and Sp1 site were also shown to be major contributors to constitutive activity. Finally, high constitutive activity of a deleted construct (-420 to -1) in NB41A3 and BHK cells suggests transcription initiates upstream of -420 in the absence of VP16. The implications of these observations regarding ICPO expression during the virus life-cycle are discussed.
来自单纯疱疹病毒1型ICPO转录起始位点-420至-70的序列对于从潜伏期重新激活并非必需。该区域外的一个假定的环磷酸腺苷反应元件（CRE）在小鼠神经母细胞瘤（NB41A3）和大鼠嗜铬细胞瘤（PC12）细胞中均无功能。此外，还观察到环磷酸腺苷反应元件结合蛋白（CREB）的结合能力较差。-95至-37的序列对于NB41A3、PC12和幼仓鼠肾（BHK）细胞的组成型活性很重要。TATA盒和Sp1位点也被证明是组成型活性的主要贡献者。最后，缺失构建体（-420至-1）在NB41A3和BHK细胞中的高组成型活性表明，在没有VP16的情况下，转录起始于-420上游。讨论了这些观察结果对病毒生命周期中ICPO表达的影响。
