Maitland M E, Leonard T, Frank C B, Shrive N G, Herzog W
Sport Medicine Centre, Human Performance Laboratory, University of Calgary, Alberta, Canada.
J Orthop Res. 1998 Jul;16(4):441-7. doi: 10.1002/jor.1100160408.
The purpose of this study was to develop a method to prospectively quantify passive knee stability in an animal model of joint injury over time. Knee stability is defined here as the amount of translation or rotation of the tibia relative to the femur for a given application of force or moment, respectively. Five animals that had undergone transection of the anterior cruciate ligament and three control animals that had undergone a sham operation were anaesthetized and positioned in a stereotaxic frame. Motion of the tibia relative to the femur was quantified with use of reflective markers secured to modified bone pins and a three-dimensional motion analysis system. External forces and moments in the transverse plane of the tibia were measured with use of force transducers based on a strain-gauge design. Longitudinal measurements of knee stability were made before either sham surgery (control animals) or transection of the ligament (experimental animals), immediately after surgery, and at 2 and 4 months after transection. The results showed that the animals tolerated the procedures well and that systematic measurements could be obtained. The method described here has the practical advantage over cross-sectional experimental designs in that the number of subjects can be decreased while maintaining statistical power and has the further conceptual advantage that individual changes can be accounted for over time.
本研究的目的是开发一种方法，用于前瞻性地量化关节损伤动物模型中随时间变化的被动膝关节稳定性。此处将膝关节稳定性定义为在给定的力或力矩作用下，胫骨相对于股骨的平移或旋转量。对5只接受前交叉韧带切断术的动物和3只接受假手术的对照动物进行麻醉，并将其放置在立体定位框架中。使用固定在改良骨针上的反光标记和三维运动分析系统对胫骨相对于股骨的运动进行量化。基于应变片设计，使用力传感器测量胫骨横平面内的外力和力矩。在假手术（对照动物）或韧带切断术（实验动物）之前、手术后立即以及韧带切断术后2个月和4个月进行膝关节稳定性的纵向测量。结果表明，动物对这些操作耐受性良好，并且可以获得系统的测量结果。与横断面实验设计相比，此处描述的方法具有实际优势，即可以减少受试者数量，同时保持统计效力，并且具有进一步的概念优势，即可以考虑个体随时间的变化。
