在豚尾猕猴外周血单核细胞（PBMCs）中的体外HIV-1感染在逆转录之后的一个步骤被阻断。
In vitro HIV-1 infection in Macaca nemestrina PBMCs is blocked at a step beyond reverse transcription.
作者信息
Kimball L E, Bosch M L
机构信息
Department of Pathobiology, University of Washington, Seattle 98195, USA.
出版信息
J Med Primatol. 1998 Apr-Jun;27(2-3):99-103. doi: 10.1111/j.1600-0684.1998.tb00233.x.
Various stages in the lifecycle of HIV-1 were investigated in Macaca nemestrina and humans in vitro. Early events were analyzed by end point dilution DNA PCR with HIV-1 and SHIV infected PBMCs, while p24 and p27 ELISA assays were used to analyze core antigen production from infected cells. The results demonstrate that a step in the virus life cycle, beyond reverse transcription is blocked for HIV-1 infection in macaque cells.
在猕猴和人体外对HIV-1生命周期的各个阶段进行了研究。通过对HIV-1和SHIV感染的外周血单核细胞进行终点稀释DNA聚合酶链反应（PCR）分析早期事件，同时使用p24和p27酶联免疫吸附测定（ELISA）分析受感染细胞产生的核心抗原。结果表明，在猕猴细胞中，HIV-1感染在病毒生命周期中逆转录之后的一个步骤被阻断。
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