黄色黏球菌脂肪酸含量的生长培养基依赖性调控由esg基因座控制。
Growth medium-dependent regulation of Myxococcus xanthus fatty acid content is controlled by the esg locus.
作者信息
Bartholomeusz G, Zhu Y, Downard J
机构信息
Department of Botany and Microbiology, University of Oklahoma, Norman, Oklahoma 73019-0245, USA.
出版信息
J Bacteriol. 1998 Oct;180(19):5269-72. doi: 10.1128/JB.180.19.5269-5272.1998.
We compared the cellular fatty acid profiles of Myxococcus xanthus cells grown in either a Casitone-based complex medium or a chemically defined medium. The cells grown in the complex medium had a much higher content of the abundant branched-chain fatty acid iso-15:0 and several other branched-chain species. The higher branched-chain fatty acid content of the cells grown in the complex medium was dependent on the esg locus, which encodes the E1alpha and E1beta components of a branched-chain keto acid dehydrogenase (BCKAD) multienzyme complex involved in branched-chain fatty acid biosynthesis. Cells grown in the complex medium were also found to have a higher level of esg transcription and more BCKAD enzyme activity than cells from the chemically defined medium. The level of esg transcription appears to be an important factor in the growth medium-dependent regulation of the M. xanthus branched-chain fatty acid content.
我们比较了在基于酪蛋白胨的复合培养基或化学成分确定的培养基中生长的黄色粘球菌细胞的细胞脂肪酸谱。在复合培养基中生长的细胞含有丰富的支链脂肪酸异-15:0和其他几种支链脂肪酸，其含量要高得多。在复合培养基中生长的细胞中较高的支链脂肪酸含量取决于esg基因座，该基因座编码参与支链脂肪酸生物合成的支链酮酸脱氢酶（BCKAD）多酶复合物的E1α和E1β组分。还发现，与在化学成分确定的培养基中生长的细胞相比，在复合培养基中生长的细胞具有更高水平的esg转录和更多的BCKAD酶活性。esg转录水平似乎是黄色粘球菌支链脂肪酸含量依赖于生长培养基调控的一个重要因素。
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