Turecký L, Kalina P, Uhlíková E, Procházková L
Ustav lekárskej chémie, biochémie a klinickej biochémie Lekárskej fakulty Univerzity Komenského v Bratislave.
Bratisl Lek Listy. 1998 Jul;99(7):347-50.
Many neurological diseases are connected with the dysfunction of blood-CSF barrier. The quantitative determination of CSF proteins has already been used in the diagnosis of barrier impairments and inflammatory diseases of the central nervous system.
Serum and CSF, totaling 264 samples, were obtained from 15 controls and 117 patients with various diseases of the nervous system. Laurell's electroimmunoassay was used for estimation of albumin and IgG levels in serum and CSF. CSF-protein profile was evaluated according to Reiber's graph for the evaluation of the CSF-protein profile.
The graph for the protein profile can be divided into 5 functionally different parts (1--normal range, 2, 3, 4--different types of barrier dysfunctions and 5--local humoral response in CNS without any barrier impairment). There was a good correlation of CSF-protein profiles and neurological diseases in our group of patients.
According to our results, Reiber's graph was helpful for the diagnosis of blood-CSF-barrier dysfunctions. The graph has the following advantages: a) possibility of simultaneous assessment of the functional state of blood-CSF-barrier and the inflammatory response of the CNS, b)sensitivity for the determination of pathological local IgG-production in CNS and c) minimal number of protein assays necessary.
许多神经系统疾病与血脑脊液屏障功能障碍有关。脑脊液蛋白质的定量测定已用于诊断中枢神经系统的屏障损伤和炎症性疾病。
从15名对照者和117名患有各种神经系统疾病的患者中获取了血清和脑脊液样本，共计264份。采用 Laurell 电免疫分析法测定血清和脑脊液中的白蛋白和 IgG 水平。根据赖伯图评估脑脊液蛋白质谱，以评估脑脊液蛋白质谱。
蛋白质谱图可分为5个功能不同的部分（1 - 正常范围，2、3、4 - 不同类型的屏障功能障碍，5 - 中枢神经系统局部体液反应，无任何屏障损伤）。在我们的患者组中，脑脊液蛋白质谱与神经系统疾病之间存在良好的相关性。
根据我们的结果，赖伯图有助于诊断血脑脊液屏障功能障碍。该图具有以下优点：a）能够同时评估血脑脊液屏障的功能状态和中枢神经系统的炎症反应；b）对中枢神经系统病理性局部IgG产生的检测具有敏感性；c）所需蛋白质检测数量最少。
