Stanović S, Boranić M
Institut Ruder Bosković Zavod za molekularnu medicinu, Laboratorij za eksperimentalnu hematologiju, imunologiju i onkologiju, Zagreb.
Lijec Vjesn. 1998 May;120(5):131-7.
Membrane metalloendopeptidase EC 3.4.24.11 (Enkephalinase, neutral endopeptidase, NEP) is a cellular ectoenzyme, immunophenotypically identified as the leukocyte cluster of differentiation CD10 or CALLA (common acute lymphoblastic leukemia antigen). Immunological, biochemical and molecular biology techniques have identified tis cell membrane feature in various organs: brain, cardiovascular system, lung, placenta, kidney etc. The CD10 immunophenotype is a common feature of lymphoblasts in acute lymphoid leukemia not expressing the T- or B-markers. The enzymatic activity of CD10/NEP possibly influences normal lymphocyte ontogeny by proteolytic cleavage of the regulatory peptides. The substrates of CD10/NEP in the kidneys are (see the list of abbreviations) ANP, adrenomedullin and PAMP; in the brain, the substrates are enkephalins and oxytocin; in the lung, bombesin, BLP, GRP, neuromedin C, substance P and neurokinin A; in the cardiovascular system, angiotenisin II, bradykinin and CGRP; in the gut, VIP; on the neutrophil membrane, fMLP etc. Some substrates are not strictly tissue-specific, e.g. substance P. Preclinical and clinical trials explore possibilities of therapeutic application of the inhibitors of neutral endopeptidase, such as thiorphan in the management of pain, diarrhoea, depression, arterial hypertension and asthma. Other possibilities of application include the treatment of hyalinomembranous disease and prevention of neurotoxicosis in tetanus and botulism.
膜金属内肽酶EC 3.4.24.11（脑啡肽酶、中性内肽酶、NEP）是一种细胞外酶，免疫表型上被鉴定为白细胞分化簇CD10或CALLA（普通急性淋巴细胞白血病抗原）。免疫学、生物化学和分子生物学技术已在各种器官中鉴定出这种细胞膜特征：脑、心血管系统、肺、胎盘、肾等。CD10免疫表型是急性淋巴细胞白血病中未表达T或B标志物的淋巴母细胞的共同特征。CD10/NEP的酶活性可能通过对调节肽的蛋白水解切割影响正常淋巴细胞的个体发生。CD10/NEP在肾脏中的底物有（见缩写列表）心钠素、肾上腺髓质素和PAMP；在脑中，底物是脑啡肽和催产素；在肺中，是蛙皮素、BLP、GRP、神经介素C、P物质和神经激肽A；在心血管系统中，是血管紧张素II、缓激肽和降钙素基因相关肽；在肠道中，是血管活性肠肽；在中性粒细胞膜上，是fMLP等。一些底物并非严格的组织特异性，例如P物质。临床前和临床试验探索了中性内肽酶抑制剂的治疗应用可能性，如硫喷妥在疼痛、腹泻、抑郁、动脉高血压和哮喘治疗中的应用。其他应用可能性包括治疗透明膜病以及预防破伤风和肉毒中毒中的神经中毒。
