Kung L, Hession A O, Bracht J P
Department of Animal and Food Sciences, University of Delaware, Newark 19717-1303, USA.
J Dairy Sci. 1998 Aug;81(8):2251-6. doi: 10.3168/jds.S0022-0302(98)75804-7.
We studied the effects of sulfur and 9,10-anthraquinone on in vitro ruminal fermentation and production of hydrogen sulfide. A complete, pelleted diet containing 26.8% acid detergent fiber, 15.9% crude protein, and 0.25 to 0.29% sulfur was used as the basal substrate. Fermentations were conducted using the basal substrate and various sulfur additions (elemental sulfur, thiosulfate, calcium sulfate, and sodium sulfate) with or without varying amounts of 9,10-anthraquinone. An increase in the sulfur content of the substrate to > 1.0% with various sources of sulfur had minimal effects on concentrations of volatile fatty acids, but the production of hydrogen sulfide increased. High amounts of 9,10-anthraquinone (10 and 25 ppm of fluid) decreased the molar proportion of acetate and decreased the production of methane and hydrogen sulfide. However, 9,10-anthraquinone increased the molar proportions of propionate and butyrate. Approximately 70% of 9,10-anthraquinone was recovered after 24 h of in vitro ruminal fermentation. These findings suggest that 9,10-anthraquinone has the potential to reduce the production of methane and hydrogen sulfide in ruminal fermentations.
我们研究了硫和9,10 - 蒽醌对体外瘤胃发酵及硫化氢产生的影响。一种含有26.8%酸性洗涤纤维、15.9%粗蛋白和0.25%至0.29%硫的完整颗粒饲料被用作基础底物。使用基础底物以及添加不同硫源（元素硫、硫代硫酸盐、硫酸钙和硫酸钠）且添加或不添加不同量9,10 - 蒽醌进行发酵。底物中硫含量增加至>1.0%，使用各种硫源时，对挥发性脂肪酸浓度影响极小，但硫化氢的产生量增加。高量的9,10 - 蒽醌（10和25 ppm液体）降低了乙酸的摩尔比例，并减少了甲烷和硫化氢的产生。然而，9,10 - 蒽醌增加了丙酸和丁酸的摩尔比例。体外瘤胃发酵24小时后，约70%的9,10 - 蒽醌被回收。这些发现表明，9,10 - 蒽醌有潜力减少瘤胃发酵中甲烷和硫化氢的产生。
