Ameye L, Compère P, Dille J, Dubois P
Laboratoire de Biologie marine, Université Libre de Bruxelles, Belgium.
Histochem Cell Biol. 1998 Sep;110(3):285-94. doi: 10.1007/s004180050290.
The ultrastructure and cytochemistry of skeleton formation sites prior to mineralization are described for the first time in echinoderms. These early sites are intracellular vacuoles located in syncytial pseudopodia of skeleton-forming cells. They contain a mineralization organic matrix, which shows a calcium-binding ability and is framed in a tridimensional structure made of concentric layers bridged by radial threads. This organic matrix presents repetitive structures which could be implicated in mineralization control. Both the tridimensional organization of the organic matrix and its framing, before mineralization starts, question the current theories which suggest that the echinoderm organic matrix is soluble at the onset of mineralization and adsorbs on the forming crystal.
首次在棘皮动物中描述了矿化之前骨骼形成部位的超微结构和细胞化学。这些早期部位是位于骨骼形成细胞合胞体伪足内的细胞内液泡。它们含有矿化有机基质，该基质具有钙结合能力，并呈三维结构，由径向线桥接的同心层构成。这种有机基质呈现出可能与矿化控制有关的重复结构。在矿化开始之前，有机基质的三维组织及其构架对当前理论提出了质疑，这些理论认为棘皮动物有机基质在矿化开始时是可溶的，并吸附在正在形成的晶体上。
