Sclavi B, Woodson S, Sullivan M, Chance M, Brenowitz M
Department of Physiology and Biophysics, Albert Einstein College of Medicine of Yeshiva University, Bronx, New York 10461, USA.
Methods Enzymol. 1998;295:379-402. doi: 10.1016/s0076-6879(98)95050-9.
The rapid mixing synchrotron X-ray footprinting technique described in this article allows nucleic acid folding and ligand binding reactions to be followed on a millisecond time resolution with single nucleotide resolution. In principle, the change in .OH protection of every nucleotide in a nucleic acid hundreds of nucleotides long can be monitored separately. In addition, a wide range of solution conditions are compatible with the radiolytic generation of .OH. These characteristics of synchrotron X-ray footprinting create opportunities for conducting thermodynamic and kinetic studies of nucleic acids that are both comprehensive and detailed. Kinetic footprinting studies of a number of systems have been initiated by the Center for Synchrotron Biosciences using this technique.
本文所述的快速混合同步加速器X射线足迹技术能够以毫秒级时间分辨率和单核苷酸分辨率追踪核酸折叠及配体结合反应。原则上，对于一条长达数百个核苷酸的核酸中每个核苷酸的·OH保护变化都能进行单独监测。此外，多种溶液条件都与·OH的辐射分解生成相兼容。同步加速器X射线足迹的这些特性为开展全面且详细的核酸热力学和动力学研究创造了机会。同步加速器生物科学中心已利用该技术启动了多个系统的动力学足迹研究。
