Yazdankhah S P, Olsen E
Department of Pharmacology, Microbiology and Food Hygiene, Norwegian College of Veterinary Medicine, Oslo, Norway.
Lett Appl Microbiol. 1998 Aug;27(2):111-5. doi: 10.1046/j.1472-765x.1998.00395.x.
A tube coagulase test (TCT) is described as a simple and non-expensive system for detection of Staphylococcus aureus directly in milk. The procedure is characterized by mixing milk samples with rabbit citrate plasma followed by incubation at 37 degrees C for clot formation. The tube coagulase test demonstrated 91.5% accuracy, 88.5% sensitivity and 100% specificity for the direct recognition of Staph. aureus in milk samples from quarters with subclinical mastitis, when compared with plating of milk on blood agar. The TCT has the potential to detect other coagulase positive staphylococci in milk. It is concluded that TCT may be of use to veterinary practitioners with limited laboratory facilities, or to dairy farmers as a simple diagnostic test on site.
试管凝固酶试验（TCT）被描述为一种直接检测牛奶中金黄色葡萄球菌的简单且低成本的系统。该方法的特点是将牛奶样品与兔柠檬酸盐血浆混合，然后在37℃下孵育以形成凝块。与将牛奶接种于血琼脂平板相比，试管凝固酶试验在直接识别患有亚临床乳腺炎乳区的牛奶样品中的金黄色葡萄球菌时，显示出91.5%的准确度、88.5%的灵敏度和100%的特异性。TCT有潜力检测牛奶中其他凝固酶阳性葡萄球菌。得出的结论是，TCT可能对实验室设施有限的兽医从业者有用，或者对奶农而言可作为一种现场简单诊断测试。
