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[卵母细胞运输(体外受精运输)对体外受精和胚胎移植程序有效性的影响]

[Effect of oocyte transportation (IVF transport) on effectiveness of the IVF and ET program].

作者信息

Drbohlav P, Hüttelová R, Rezácová J, Masata J, Mardesic T

机构信息

I. gynek.-porod. klinika 1. LF UK a VFN, Praha.

出版信息

Ceska Gynekol. 1998 Aug;63(4):301-5.

PMID:9750405
Abstract

The programme of in vitro fertilization helps at present an ever increasing number of married couples to resolve successfully the problem of sterility. It is however a method pretentions technically, as regards time, material and in particular funds. This applies above all to embryological laboratories. The transport system of the IVF programme makes possible care of patients indicated for sterility treatment by the IVF method in departments without their own embryological laboratory and thus it is possible to include more patients in the programme and save money. In the author's department the first two stages of the IVF programme are implemented, i.e. stimulation of the ovaries and aspiration of follicular fluid. Then this material is taken in a transport box of the K-system Co., Denmark, type G-81E to a centre with full accreditation where the subsequent stages of the IVF programme take place (selection of the oocyte, fertilization, cultivation of embryos and embryo transfer). The transport system has operated now for three years (1995-1997) and at present the authors evaluate the system. The fertility rate and pregnancy rate in individual years was compared and the following conclusions were reached: The fertility rate is significantly (at the 1% level) lower in the transport centre in 1995 and 1996 (47%, 54%) as compared with the other centre the laboratory of which is used by the authors (57%, 61%). In 1997 the difference is no longer significant (63% x 64%). The difference in the pregnancy rate in centres for aspiration and embryo transfer in both centres is insignificant in the years of investigation. It may thus be stated that the oocyte transport does not have a negative influence on the effectiveness of the IVF + ET programme (same pregnancy rate) and thus makes it possible to include a greater and ever increasing number of patients in the IVF programme by making use of reserve capacities of embryological laboratories and thus reduce the total costs of this programme.

摘要

目前,体外受精方案帮助越来越多的已婚夫妇成功解决不育问题。然而,该方法在技术上对时间、材料,尤其是资金要求很高。这一点在胚胎学实验室方面尤为突出。体外受精方案的运输系统使得在没有自身胚胎学实验室的科室中,能够对适合采用体外受精方法进行不育治疗的患者进行护理,从而有可能让更多患者参与该方案并节省资金。在作者所在科室实施体外受精方案的前两个阶段,即卵巢刺激和卵泡液抽吸。然后,将这些材料装入丹麦K系统公司的G - 81E型运输箱,运至一个完全认可的中心,在那里进行体外受精方案的后续阶段(卵母细胞选择、受精、胚胎培养和胚胎移植)。该运输系统现已运行三年(1995 - 1997年),目前作者对该系统进行评估。比较了各年的受精率和妊娠率,得出以下结论:1995年和1996年,运输中心的受精率(47%,54%)与作者使用其实验室的另一个中心相比显著较低(1%水平)(57%,61%)。1997年,差异不再显著(63%对64%)。在调查年份中,两个中心的卵泡液抽吸和胚胎移植中心的妊娠率差异不显著。因此可以说,卵母细胞运输对体外受精 + 胚胎移植方案的有效性没有负面影响(妊娠率相同),从而通过利用胚胎学实验室的备用能力,有可能让越来越多的患者参与体外受精方案,进而降低该方案的总成本。

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