Adam Z, Klabusay M, Vorlícek J, Hájek R
II. interní klinika FNsP, Brno Bohunice.
Vnitr Lek. 1997 Sep;43(9):592-8.
Bone marrow plasma cells from patients with multiple myeloma express monoclonal cytoplasmic immunoglobulin, strongly express CD38 and usually coexpress CD56 and CD54. The aim of this study was to learn the relationship between the number of CD38+CD56+ and CD38+CD54 positive cells in peripheral blood with the activity of multiple myeloma. We evaluated the number of these cells in patients with monoclonal gammopathy and in the control group, as well. We used the two-color flow cytometry for this purpose. The peripheral blood of 57 patients with multiple myeloma and 4 patients with monoclonal gammopathy of unknown significance was repeatedly analyzed. Patients with high activity of multiple myeloma had the highest percentage of CD38+CD56+ and CD38+CD54+ cells. Patients with lower activity of the disease had lower count of these cells. But in 4 (11%) patients with high disease activity the count of CD38+CD56+ cells was zero. We conclude, that the longitudinal flow cytometric analysis of CD38+CD54+ and CD38+CD56+ cells is a useful method for estimation of disease activity in patients with multiple myeloma. Single analysis of these parameters cannot be used as a strict criterium for differential diagnosis.
多发性骨髓瘤患者的骨髓浆细胞表达单克隆细胞质免疫球蛋白，强烈表达CD38，通常还共表达CD56和CD54。本研究的目的是了解外周血中CD38+CD56+和CD38+CD54阳性细胞数量与多发性骨髓瘤活性之间的关系。我们还评估了单克隆丙种球蛋白病患者和对照组中这些细胞的数量。为此我们使用了双色流式细胞术。对57例多发性骨髓瘤患者和4例意义未明的单克隆丙种球蛋白病患者的外周血进行了反复分析。多发性骨髓瘤活性高的患者中CD38+CD56+和CD38+CD54+细胞的百分比最高。疾病活性较低的患者这些细胞的计数较低。但在4例（11%）疾病活性高的患者中，CD38+CD56+细胞计数为零。我们得出结论，对CD38+CD54+和CD38+CD56+细胞进行纵向流式细胞术分析是评估多发性骨髓瘤患者疾病活性的一种有用方法。对这些参数进行单次分析不能用作鉴别诊断的严格标准。
