Fenselau A, Mello R J
Cancer Res. 1976 Sep;36(9 pt.1):3269-73.
Short-term cultures of endothelial cells from fetal bovine heart and aorta consistently display increased growth rates when crude tumor cell homogenates from the Walker 256 adenocarcinoma (ascites and solid forms as well as tumor cells from a suspension culture) are added to the culture media. The tumor-derived material produces growth stimulation in both sparse and confluient endothelial cell cultures. Homogenates of embryonic tissues and cultured cells show similar growth-promoting effects; corresponding material from various adult tissues is ineffective. The nonendothelial cells that were tested generally show no growth response or only a slight positive growth response to the tumor cell homogenates. The results indicate the feasibility of using this in vitro system as a paradigm of the tumor-induced vascularization process.
当将来自Walker 256腺癌的粗制肿瘤细胞匀浆（腹水和实体瘤形式以及悬浮培养的肿瘤细胞）添加到培养基中时，来自胎牛心脏和主动脉的内皮细胞短期培养物始终显示出增加的生长速率。肿瘤来源的物质在稀疏和汇合的内皮细胞培养物中均产生生长刺激作用。胚胎组织和培养细胞的匀浆显示出类似的促生长作用；来自各种成年组织的相应物质则无效。所测试的非内皮细胞通常对肿瘤细胞匀浆没有生长反应或只有轻微的阳性生长反应。结果表明，使用这种体外系统作为肿瘤诱导血管生成过程范例的可行性。
