Lingfei K, Pingzhang Y, Zhengguo L, Jianhua G, Yaowu Z
Department of Pathology, Henan Provincial Hospital, Zheng Zhou, People's Republic of China.
Cancer Lett. 1998 Aug 14;130(1-2):93-101. doi: 10.1016/s0304-3835(98)00115-3.
To observe the expression of p16, pRb, cdk4 and cyclinD1 in non-small cell lung cancers, 104 cases of resected lung cancers were collected, which included squamous cell carcinomas, adenocarcinomas and large cell carcinomas. Immunohistochemistry assay was carried out. The results showed that 67% of squamous cell carcinomas and 46% of adenocarcinomas expressed p16, 64% of squamous cell carcinomas and 85% of adenocarcinomas expressed pRb and 66% of cancers expressed p16 or pRb. About 70% of the tumors expressed cyclinD1. More than 90% of the tumors expressed cdk4 and there was an increased trend with decreasing differentiation of both squamous cell carcinomas and adenocarcinomas. Sixty-seven percent of the highly differentiated and 100% of the poorly differentiated squamous cell carcinomas expressed cdk4. The aberrant p16 and pRb gene product expression played a significant role in the development and histological subtype of lung cancers by conditioning the biological behavior of NSCLC. cdk4 was an important factor in histological differentiation.
为观察非小细胞肺癌中p16、pRb、cdk4和细胞周期蛋白D1的表达情况，收集了104例手术切除的肺癌病例，其中包括鳞状细胞癌、腺癌和大细胞癌。进行了免疫组织化学检测。结果显示，67%的鳞状细胞癌和46%的腺癌表达p16，64%的鳞状细胞癌和85%的腺癌表达pRb，66%的癌症表达p16或pRb。约70%的肿瘤表达细胞周期蛋白D1。超过90%的肿瘤表达cdk4，且随着鳞状细胞癌和腺癌分化程度降低有增加趋势。67%的高分化和100%的低分化鳞状细胞癌表达cdk4。p16和pRb基因产物的异常表达通过调节非小细胞肺癌的生物学行为，在肺癌的发生发展和组织学亚型中起重要作用。cdk4是组织学分化中的一个重要因素。
