Faure-Virelizier C, Croix D, Bouret S, Prévot V, Reig S, Beauvillain J C, Mitchell V
Neuroendocrinologie et Physiopathologie Neuronale, INSERM U-422, Lille, France.
Endocrinology. 1998 Oct;139(10):4127-39. doi: 10.1210/endo.139.10.6271.
Variations in the number of galanin receptor (Gal-R1)-expressing cells and levels of Gal-R1 messenger RNA (mRNA) were determined in the preoptic area in intact female rats throughout the phases of the estrous cycle and compared with those in the male. Female and male Wistar rats were fixed by perfusion with 4% paraformaldehyde. Cryostat sections were hybridized with a 35S-labeled antisense Gal-R1 riboprobe. The number of Gal-R1 mRNA-expressing cells was lower in the rostral preoptic area than in the medial preoptic area. During the estrous cycle, the highest number of Gal-R1 mRNA-expressing cells in the rostral preoptic region was detected at 0800 h on proestrus, whereas in the medial preoptic area, the maximum number was observed at 1800 h on estrus. Gal-R1 mRNA levels in individual cells were low during diestrus and increased at estrus in both areas. In the male, the number of mRNA-expressing cells and the hybridization signal were significantly lower than those in females during estrus. The results demonstrate that Gal-R1 gene expression in the preoptic area varies during the estrous cycle and is low in males. Short term treatment of ovariectomized rats with estradiol plus progesterone caused significantly decreased preoptic Gal-R1 mRNA levels compared with those after treatment with estrogen only. These observations suggest that in the preoptic area, expression of Gal-R1 is influenced by progesterone. The variation in Gal-R1 expression is likely to influence the extent to which galanin can influence the preoptic cells implicated in the control of neighboring GnRH cells.
在完整雌性大鼠发情周期的各个阶段，测定视前区中表达甘丙肽受体（Gal-R1）的细胞数量和Gal-R1信使核糖核酸（mRNA）水平，并与雄性大鼠进行比较。雌性和雄性Wistar大鼠通过灌注4%多聚甲醛进行固定。冷冻切片与35S标记的反义Gal-R1核糖探针杂交。视前区头端表达Gal-R1 mRNA的细胞数量低于视前区内侧。在发情周期中，视前区头端在发情前期08:00时检测到表达Gal-R1 mRNA的细胞数量最多，而在视前区内侧，发情期18:00时观察到细胞数量最多。在间情期，两个区域单个细胞中的Gal-R1 mRNA水平较低，在发情期升高。在雄性中，发情期表达mRNA的细胞数量和杂交信号明显低于雌性。结果表明，视前区中Gal-R1基因表达在发情周期中有所变化，且在雄性中较低。与仅用雌激素处理后相比，用雌二醇加孕酮对去卵巢大鼠进行短期处理会导致视前区Gal-R1 mRNA水平显著降低。这些观察结果表明，在视前区，Gal-R1的表达受孕酮影响。Gal-R1表达的变化可能会影响甘丙肽对与邻近促性腺激素释放激素（GnRH）细胞控制有关的视前细胞的影响程度。
