Ruble D M, Foster D N
University of Minnesota, Division of Animal Physiology, Department of Animal Science, St. Paul, MN 55108, USA.
Gene. 1998 Sep 18;218(1-2):95-101. doi: 10.1016/s0378-1119(98)00383-7.
A chicken cellular nucleic acid binding protein (cCNBP) cDNA was isolated from a chicken Con-A-stimulated immune cell library by differential screening. cCNBP is a Cys/Cys-His/Cys zinc finger DNA binding protein of unknown function. The chicken CNBP nucleotide and deduced amino acid sequence showed extraordinary sequence conservation (between 81-98% similarities) when compared to human, mouse and rat CNBP. The CNBP gene was shown to be a single copy and to cross-hybridize to human and mouse genomic DNA. A Northern blot analysis revealed cCNBP to be a constitutively expressed gene in a wide variety of tissues and to be differentially expressed in cultured chicken spleen and bursal cells after mitogen stimulation.
通过差异筛选从鸡Con - A刺激的免疫细胞文库中分离出一种鸡细胞核酸结合蛋白（cCNBP）cDNA。cCNBP是一种功能未知的Cys/Cys - His/Cys锌指DNA结合蛋白。与人类、小鼠和大鼠的CNBP相比，鸡CNBP的核苷酸和推导的氨基酸序列显示出极高的序列保守性（相似性在81 - 98%之间）。CNBP基因显示为单拷贝，并且能与人及小鼠基因组DNA发生交叉杂交。Northern印迹分析表明，cCNBP是一种在多种组织中组成性表达的基因，并且在有丝分裂原刺激后，在培养的鸡脾脏和法氏囊细胞中差异表达。
