Macey J R, Schulte J A, Ananjeva N B, Larson A, Rastegar-Pouyani N, Shammakov S M, Papenfuss T J
Department of Biology, Washington University, St. Louis, Missouri 63130-4899, USA.
Mol Phylogenet Evol. 1998 Aug;10(1):118-31. doi: 10.1006/mpev.1997.0478.
Phylogenetic relationships within the Laudakia caucasia species group on the Iranian Plateau were investigated using 1708 aligned bases of mitochondrial DNA sequence from the genes encoding ND1 (subunit one of NADH dehydrogenase), tRNAGln, tRNAIle, tRNAMet, ND2, tRNATrp, tRNAAla, tRNAAsn, tRNACys, tRNATyr, and COI (subunit I of cytochrome c oxidase). The aligned sequences contain 207 phylogenetically informative characters. Three hypotheses for historical fragmentation of Laudakia populations on the Iranian Plateau were tested. In two hypotheses, fragmentation of populations is suggested to have proceeded along continuous mountain belts that surround the Iranian Plateau. In another hypothesis, fragmentation is suggested to have resulted from a north-south split caused by uplifting of the Zagros Mountains in the late Miocene or early Pliocene [5-10 MYBP (million years before present)]. The shortest tree suggest the later hypothesis, and statistical tests reject the other two hypothesis. The phylogenetic tree is exceptional in that every branch is well supported. Geologic history provides dates for most branches of the tree. A plot of DNA substitutions against dates from geologic history refines the date for the north-south split across the Iranian Plateau to 9 MYBP (late Miocene). The rate of evolution for this segment of mtDNA is 0.65% (0.61-0.70%) change per lineage per million years. A hypothesis of area relationships for the biota of the Iranian Plateau is generated from the phylogenetic tree.
利用编码 ND1（烟酰胺腺嘌呤二核苷酸脱氢酶亚基一）、tRNAGln、tRNAIle、tRNAMet、ND2、tRNATrp、tRNAAla、tRNAAsn、tRNACys、tRNATyr 和 COI（细胞色素 c 氧化酶亚基 I）的线粒体 DNA 序列的 1708 个比对碱基，研究了伊朗高原上高加索岩蜥物种组内的系统发育关系。比对序列包含 207 个系统发育信息特征。对伊朗高原上高加索岩蜥种群历史片段化的三种假说进行了检验。在两种假说中，种群片段化被认为是沿着环绕伊朗高原的连续山脉带进行的。在另一种假说中，片段化被认为是由中新世晚期或上新世早期（距今 500 - 1000 万年）扎格罗斯山脉隆升导致的南北分裂造成的。最短树支持后一种假说，统计检验拒绝了其他两种假说。该系统发育树的特殊之处在于每个分支都得到了很好的支持。地质历史为树的大多数分支提供了时间。将 DNA 替代与地质历史时间作图，将伊朗高原南北分裂的时间精确到距今 900 万年（中新世晚期）。这段线粒体 DNA 的进化速率为每谱系每百万年 0.65%（0.61 - 0.70%）的变化。从系统发育树中得出了关于伊朗高原生物区系区域关系的假说。
