Gilles A, Lecointre G, Faure E, Chappaz R, Brun G
Laboratoire d'Hydrobiologie, Université de Provence, Marseille, France.
Mol Phylogenet Evol. 1998 Aug;10(1):132-43. doi: 10.1006/mpev.1997.0480.
Two different mitochondrial genes, the cytochrome b and the 16S rDNA, support the same European cyprinid molecular phylogeny: the most basal subfamily is the paraphyletic Rasborinae, the Cyprininae are monophyletic, the Tincinae and Gobioninae are close to the Cyprininae or more basal lineages but not close to Leuciscinae or Alburninae, and the Leuciscinae are paraphyletic but can become monophyletic if we include the biphyletic alburninae and exclude the Phoxinini. The relationship of the Acheilognathinae remains obscure. Natural intergeneric and interspecific hybridizations are clearly demonstrated within the Leuciscinae, both from high bootstrap proportions and intermediate morphological features: Chondrostoma toxostoma and Rutilus rutilus, Scardinius erythrophthalmus and R. rutilus, and Leuciscus multicellus and Leuciscus soufia. Finally, the use of the nonsaturated and clockwise 16S mtDNA sequences have been used to infer from nonintrogressive taxa the time of the first European cyprinid cladogeneses. The estimation confirms the hypothesis of Almaça and Banarescu that European cyprinid subfamilies started to diversify 35 mya and confirms the hypothesis of Bianco on the diversification of European leuciscines in the Mediterranean area during the late Messinian (6.5 to 5.3 mya).
两个不同的线粒体基因，即细胞色素b和16S rDNA，支持相同的欧洲鲤科鱼类分子系统发育：最基部的亚科是并系的波鱼亚科，鲤亚科是单系的，丁鱥亚科和鮈亚科接近鲤亚科或更基部的谱系，但不接近雅罗鱼亚科或赤梢鱼亚科，雅罗鱼亚科是并系的，但如果我们纳入双系的赤梢鱼亚科并排除麦穗鱼属，则可以成为单系的。鳑鲏亚科的关系仍然不明确。在雅罗鱼亚科中，通过高自展比例和中间形态特征清楚地证明了自然属间和种间杂交：细口突吻鱼和赤睛圆腹雅罗鱼、赤眼鳟和赤睛圆腹雅罗鱼，以及多纹雅罗鱼和索非亚雅罗鱼。最后，利用不饱和且顺时针的16S线粒体DNA序列从非渐渗类群推断出欧洲鲤科鱼类首次分支发生的时间。该估计证实了阿尔马卡和巴纳雷斯库的假说，即欧洲鲤科鱼类亚科在3500万年前开始分化，并证实了比安科关于欧洲雅罗鱼亚科在梅西尼期晚期（650万至530万年前）在地中海地区分化的假说。
