Lauri S E, Rauvala H, Kaila K, Taira T
Department of Biosciences, University of Helsinki, Finland.
Eur J Neurosci. 1998 Jan;10(1):188-94. doi: 10.1046/j.1460-9568.1998.00039.x.
Heparin-binding growth-associated molecule (HB-GAM) is an 18-kDa developmentally regulated protein, which promotes neurite outgrowth, axonal guidance and synaptogenesis through interaction with cell-surface heparan-sulphate proteoglycans. We have studied the effect of HB-GAM on synaptic transmission and long-term potentiation (LTP) in the area CA1 of rat hippocampal slices, where HB-GAM mRNA is expressed in an activity-dependent manner. Injection of recombinant HB-GAM into the dendritic area inhibited tetanus-induced LTP without affecting baseline synaptic responses or the N-methyl-D-aspartate (NMDA)-receptor mediated transmission. HB-GAM did not depotentiate tetanus-induced LTP or prevent heterosynaptic LTP induced by application of tetraethylammonium (TEA), indicating that the effect was limited to early, synapse-specific stages of LTP induction. These results suggest that HB-GAM is involved in the regulation of synaptic plasticity in hippocampus.
肝素结合生长相关分子（HB-GAM）是一种18 kDa的受发育调控的蛋白质，它通过与细胞表面硫酸乙酰肝素蛋白聚糖相互作用促进神经突生长、轴突导向和突触形成。我们研究了HB-GAM对大鼠海马切片CA1区突触传递和长时程增强（LTP）的影响，在该区域HB-GAM mRNA以活动依赖的方式表达。将重组HB-GAM注射到树突区域可抑制强直刺激诱导的LTP，而不影响基线突触反应或N-甲基-D-天冬氨酸（NMDA）受体介导的传递。HB-GAM不会使强直刺激诱导的LTP去增强，也不会阻止应用四乙铵（TEA）诱导的异突触LTP，这表明该效应仅限于LTP诱导的早期、突触特异性阶段。这些结果表明，HB-GAM参与海马突触可塑性的调节。
