Aihara M, Imagawa K, Funakoshi Y, Ohmoto Y, Kikuchi M
Microbiological Research Institute, Otsuka Pharmaceutical Co. Ltd., Tokushima, Japan.
Dig Dis Sci. 1998 Sep;43(9 Suppl):160S-166S.
Recently, the relative contributions of local T helper cell responses of the Th1-type and Th2-type to the pathogenesis of gastritis and peptic ulcers associated with Helicobacter pylori infection have been examined. However, the results were controversial with respect to whether cellular immunity (Th1-type) or humoral immunity (Th2-type) responses predominate in H. pylori infection and with respect to how these responses may contribute to disease pathogenesis. In this study, we investigated the characteristics of the production of various cytokines induced by H. pylori or lipopolysaccharide (LPS), which was derived from H. pylori or Escherichia coli, in human peripheral blood mononuclear cells (PBMC). Live H. pylori induced production of many cytokines, such as IL-1beta, IL-10, IL-8, IFN-gamma, and TNF-alpha, whereas we could not detect IL-2 or IL-4. Moreover, we evaluated the effect of rebamipide on the production of several cytokines from PBMC induced by various stimuli. Rebamipide suppressed the production of IL-8, IL-10, TNF-alpha, and IL-1beta induced by H. pylori in a dose-dependent manner. On the other hand, the production of IL-12 induced by H. pylori showed a tendency to increase as a result of treatment of the cells with rebamipide. These results suggested that rebamipide might be effective in regulating cytokine responses in the H. pylori-infected host and maintaining host immunity. Moreover, it might contribute positively to disease progression and bacterial eradication.
最近，已经研究了Th1型和Th2型局部T辅助细胞反应对幽门螺杆菌感染相关的胃炎和消化性溃疡发病机制的相对贡献。然而，关于细胞免疫（Th1型）或体液免疫（Th2型）反应在幽门螺杆菌感染中占主导地位，以及这些反应如何促进疾病发病机制，结果存在争议。在本研究中，我们调查了幽门螺杆菌或脂多糖（LPS，源自幽门螺杆菌或大肠杆菌）在人外周血单核细胞（PBMC）中诱导产生的各种细胞因子的特征。活的幽门螺杆菌诱导产生许多细胞因子，如IL-1β、IL-10、IL-8、IFN-γ和TNF-α，而我们检测不到IL-2或IL-4。此外，我们评估了瑞巴派特对各种刺激诱导的PBMC中几种细胞因子产生的影响。瑞巴派特以剂量依赖的方式抑制幽门螺杆菌诱导的IL-8、IL-10、TNF-α和IL-1β的产生。另一方面，幽门螺杆菌诱导的IL-12的产生在细胞用瑞巴派特处理后有增加的趋势。这些结果表明，瑞巴派特可能在调节幽门螺杆菌感染宿主的细胞因子反应和维持宿主免疫方面有效。此外，它可能对疾病进展和细菌根除有积极作用。
