Sugiyama A, Ishida K, Ikeno T, Maruta F, Kawasaki S, Akamatsu T, Katsuyama T
First Department of Surgery, Shinshu University School of Medicine, Matsumoto, Japan.
Dig Dis Sci. 1998 Sep;43(9 Suppl):188S-191S.
This study was performed to distinguish between the coccoid form or spiral forms of Helicobacter pylori (Hp) and to elucidate the pathologic significance of these shapes of Hp. Specimens obtained from human or Mongolian gerbil stomachs were fixed in Carnoy's solution and embedded in paraffin. Sections 3 or 10 microm thick were stained with polyclonal anti-Hp antibody by the immunoperoxidase method. Stereo pairs were prepared from these thick sections by computer-assisted reconstruction. The two shapes of Hp were easily distinguishable by this method. In the human stomach, the proportions of Hp in the surface mucous gel layer (SMGL) and the gastric pits were 31.5% and 68.5%, and the percentage of the spiral form varied from 31.9% to 66.3%. In Mongolian gerbils, a higher proportion of Hp colonized the SMGL, and the spiral form existed more frequently both in the SMGL and on the surfaces of the surface mucous cells.
本研究旨在区分幽门螺杆菌（Hp）的球菌形态或螺旋形态，并阐明这些形态的Hp的病理学意义。从人或蒙古沙鼠胃中获取的标本用卡诺氏固定液固定，然后石蜡包埋。将3微米或10微米厚的切片用免疫过氧化物酶法进行多克隆抗Hp抗体染色。通过计算机辅助重建从这些厚切片制备立体对。通过该方法可轻松区分Hp的两种形态。在人胃中，Hp在表面黏液凝胶层（SMGL）和胃小凹中的比例分别为31.5%和68.5%，螺旋形态的百分比在31.9%至66.3%之间变化。在蒙古沙鼠中，更高比例的Hp定殖于SMGL，并且螺旋形态在SMGL和表面黏液细胞表面均更频繁地存在。
