McAlindon M E, Gray T, Galvin A, Sewell H F, Podolsky D K, Mahida Y R
Division of Gastroenterology, University Hospital, Nottingham, England.
Gastroenterology. 1998 Oct;115(4):841-8. doi: 10.1016/s0016-5085(98)70255-0.
BACKGROUND & AIMS: In active inflammatory bowel disease (IBD), the intestinal mucosa is infiltrated by polymorphonuclear cells (PMNs), lymphocytes, and monocytes from the systemic circulation. Using an ex vivo model, we have investigated luminally directed migration of cells out of the lamina propria.
Fresh untreated and deepithelialized mucosal samples were studied by electron microscopy. Cells migrating out of the lamina propria were investigated by immunohistochemistry and fluorescence-activated cell sorter analysis.
In intact IBD mucosal samples, tunnels containing cells were prominent in the lamina propria matrix, and PMNs, but not other cell types, were prominent in the epithelium. In deepithelialized mucosal samples, the basement membrane was either destroyed or contained numerous large pores. During culture of deepithelialized mucosal samples, many cells (3.3 [+/-0.8] x 10(5) . g tissue-1 . h-1) migrated out of the lamina propria via basement membrane pores. PMNs and eosinophils were prominent during the first 3 hours of culture, but T cells predominated thereafter. Macrophages also migrated, but B cells were the minority population (<2%) at all times.
In active IBD mucosa with an intact epithelium, luminally directed migration of lamina propria cells is restricted mainly to PMNs. After loss of the epithelium, other cell types also migrate into the lumen via numerous, large, basement membrane pores.
在活动性炎症性肠病（IBD）中，肠道黏膜被来自体循环的多形核细胞（PMN）、淋巴细胞和单核细胞浸润。我们使用体外模型研究了固有层细胞向肠腔方向的迁移。
通过电子显微镜研究新鲜未处理和去上皮的黏膜样本。通过免疫组织化学和荧光激活细胞分选分析研究从固有层迁移出的细胞。
在完整的IBD黏膜样本中，固有层基质中含有细胞的通道很突出，并且PMN（而非其他细胞类型）在上皮中很突出。在去上皮的黏膜样本中，基底膜要么被破坏，要么含有许多大孔。在去上皮的黏膜样本培养过程中，许多细胞（3.3[±0.8]×10⁵·g组织⁻¹·h⁻¹）通过基底膜孔从固有层迁移出来。培养的前3小时PMN和嗜酸性粒细胞很突出，但此后T细胞占主导。巨噬细胞也会迁移，但B细胞在所有时间都是少数群体（<2%）。
在具有完整上皮的活动性IBD黏膜中，固有层细胞向肠腔方向的迁移主要限于PMN。上皮丧失后，其他细胞类型也通过大量的基底膜大孔迁移到肠腔。
