Kawabata S, Boyaka P N, Coste M, Fujihashi K, Yamamoto M, McGhee J R, Kiyono H
Departments of Oral Biology and Microbiology, Immunobiology Vaccine Center, University of Alabama at Birmingham, Birmingham, Alabama, USA.
Gastroenterology. 1998 Oct;115(4):866-73. doi: 10.1016/s0016-5085(98)70258-6.
BACKGROUND & AIMS: Intraepithelial lymphocytes (IELs) are located between epithelial cells that are thought to display unique features and functions at the small intestinal villus tip and crypt levels. We have addressed whether the spatial differences in the intestinal epithelium extend to IELs and subsequent cross-talk between IELs and epithelial cells.
IELs were isolated from villus tip and crypt portions of mouse small intestine and then compared for spontaneous cytokine production and responsiveness to interleukin (IL)-2 and/or IL-7.
No difference was observed between number of beta IELs in villus tips and crypts, whereas a trend toward increased frequencies of IELs bearing the gamma delta form of T-cell receptor was noted in villus tips. Interestingly, the number of beta IELs producing interferon gamma and IL-5 was significantly reduced in the cells from crypts compared with villus tips. Furthermore, villus tip beta IELs exhibited higher responses to stimulation signals provided by IL-2 and/or IL-7 than their crypt counterpart. Such functional differences were not observed with gamma delta IELs from the two intestinal sites.
Distinct molecular cross-talk between IELs and epithelial cells occurs in intestinal villus tips and crypts.
上皮内淋巴细胞（IELs）位于上皮细胞之间，在小肠绒毛顶端和隐窝水平被认为具有独特的特征和功能。我们探讨了肠道上皮的空间差异是否延伸至IELs，以及IELs与上皮细胞之间随后的相互作用。
从小鼠小肠的绒毛顶端和隐窝部分分离IELs，然后比较其自发细胞因子产生情况以及对白细胞介素（IL）-2和/或IL-7的反应性。
绒毛顶端和隐窝中β IELs的数量没有差异，而在绒毛顶端观察到携带γδ形式T细胞受体的IELs频率有增加的趋势。有趣的是，与绒毛顶端相比，隐窝细胞中产生干扰素γ和IL-5的β IELs数量显著减少。此外，绒毛顶端的β IELs对IL-2和/或IL-7提供的刺激信号的反应高于其隐窝对应物。来自两个肠道部位的γδ IELs未观察到这种功能差异。
IELs与上皮细胞之间在肠道绒毛顶端和隐窝中发生明显的分子相互作用。
