一种在裸云母表面上对齐大DNA分子以用于原子力显微镜的便捷方法。
A convenient method of aligning large DNA molecules on bare mica surfaces for atomic force microscopy.
作者信息
Li J, Bai C, Wang C, Zhu C, Lin Z, Li Q, Cao E
机构信息
The Institute of Chemistry, The Chinese Academy of Sciences, Beijing 100080, China and The Institute of Biophysics, the Chinese Academy of Sciences, Beijing 100171, China.
出版信息
Nucleic Acids Res. 1998 Oct 15;26(20):4785-6. doi: 10.1093/nar/26.20.4785.
Large DNA molecules remain difficult to be imaged by atomic force microscopy (AFM) because of the tendency of aggregation. A method is described to align long DNA fibers in a single direction on unmodified mica to facilitate AFM studies. The clear background, minimal overstretching, high reproducibility and convenience of this aligning procedure make it useful for physical mapping of genome regions and the studies of DNA-protein complexes.
由于存在聚集倾向，大型DNA分子仍然难以通过原子力显微镜（AFM）成像。本文描述了一种方法，可使长DNA纤维在未修饰的云母上沿单一方向排列，以促进AFM研究。这种排列方法背景清晰、过度拉伸最小、重现性高且操作方便，使其在基因组区域的物理图谱绘制和DNA-蛋白质复合物研究中很有用。
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