结晶紫对致病性血鞭毛虫鲑隐鞭虫（Cryptobia salmositica Katz，1951）（肉鞭门：动基体目）的体外作用
The in vitro effects of crystal violet on the pathogenic haemoflagellate Cryptobia salmositica Katz, 1951 (Sarcomastigophora: Kinetoplastida).
作者信息
Ardelli B F, Woo P T
机构信息
Department of Zoology, University of Guelph, Ontario, Canada.
出版信息
Parasite. 1998 Mar;5(1):27-36. doi: 10.1051/parasite/1998051027.
Crystal violet does not inhibit in vitro multiplication of a nonpathogenic strain of Cryptobia salmositica at low concentrations (0.01 microM and 0.001 microM) but multiplication is inhibited at higher concentrations (> or = 0.05 microM). In contrast, the pathogenic strain of C. salmositica does not multiply in vitro when incubated with crystal violet (0.001 microM, 0.01 microM and 0.05 microM). The infectivity of the pathogenic strain is significantly reduced after in vitro exposure to crystal violet. Crystal violet lyses C. salmositica (100.0 microM) and causes lesions on mitochondrial and nuclear membranes of the parasite. Pathogenic strains of Cryptobia salmositica and C. bullocki are more susceptible to lysis after in vitro exposure to crystal violet than are nonpathogenic strains of Cryptobia salmositica and C. catostomi.
结晶紫在低浓度（0.01微摩尔和0.001微摩尔）时不抑制鲑隐鞭虫非致病菌株的体外增殖，但在较高浓度（≥0.05微摩尔）时增殖受到抑制。相比之下，当与结晶紫（0.001微摩尔、0.01微摩尔和0.05微摩尔）一起孵育时，鲑隐鞭虫致病菌株在体外不增殖。致病菌株在体外暴露于结晶紫后，其感染力显著降低。结晶紫可裂解鲑隐鞭虫（100.0微摩尔），并在寄生虫的线粒体膜和核膜上造成损伤。与鲑隐鞭虫和鲶隐鞭虫的非致病菌株相比，鲑隐鞭虫和布氏隐鞭虫的致病菌株在体外暴露于结晶紫后对裂解更敏感。
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