Neiswanger K, Zubenko G S, Giles D E, Frank E, Kupfer D J, Kaplan B B
Department of Psychiatry, University of Pittsburgh School of Medicine, Pennsylvania, USA.
Am J Med Genet. 1998 Sep 7;81(5):443-9.
Recurrent unipolar depression with an early age of onset is a severe form of unipolar depression that has both genetic and environmental components. We genotyped the members of 16 families identified by probands with early onset (< or = 25 years), recurrent unipolar, major depression for 38 simple sequence tandem repeat polymorphisms (SSTRPs) from chromosomal regions containing 12 genes involved in neuroendocrine or serotonergic functioning. Pairwise linkage analysis was performed with the software package FASTLINK. The affected phenotype was defined four ways, and both dominant and recessive models of depression were analyzed. Seven SSTRPs showed lod scores > 1.00 at theta values between 0.10-0.20. The members of an additional 18 families were genotyped for these seven SSTRPs, and the complete sample of 34 families was evaluated using lod score analysis, affected pedigree member linkage analysis, and within-family association analysis. Evidence for linkage between D11S929 and affective illness remained positive, necessitating the analysis of four additional SSTRPs within 3 cM of D11S929. After all confirmatory analyses were completed, no evidence suggestive of linkage remained between any of the 38 SSTRPs and the affected phenotypes.
早发型复发性单相抑郁症是单相抑郁症的一种严重形式，具有遗传和环境因素。我们对16个家族的成员进行了基因分型，这些家族由先证者确定，其患有早发型（≤25岁）、复发性单相重度抑郁症，针对来自包含12个参与神经内分泌或血清素功能的基因的染色体区域的38个简单序列串联重复多态性（SSTRP）进行检测。使用FASTLINK软件包进行成对连锁分析。以四种方式定义受影响的表型，并分析了抑郁症的显性和隐性模型。七个SSTRP在θ值为0.10 - 0.20之间时显示连锁值>1.00。对另外18个家族的成员进行了这七个SSTRP的基因分型，并使用连锁值分析、受影响家系成员连锁分析和家系内关联分析对34个家族的完整样本进行了评估。D11S929与情感性疾病之间的连锁证据仍然为阳性，因此需要对D11S929附近3 cM范围内的另外四个SSTRP进行分析。在所有验证性分析完成后，38个SSTRP中的任何一个与受影响表型之间均未留下提示连锁的证据。
