El-Sayed L H, Ghoneim H, Demian S R, El-Sayed M H, Tawfik N M, Sakr I, Abou-Basha L M, Renganathan E, Klinkert M Q, Abou-Rawash N
Department of Immunology, Medical Research Institute, University of Alexandria, Egypt.
Trop Med Int Health. 1998 Sep;3(9):721-7. doi: 10.1046/j.1365-3156.1998.00298.x.
We performed a series of ELISAs to evaluate the diagnostic significance of two Schistosoma mansoni proteins, Sm31 (cysteine proteinase, cathepsin B) and Sm32 (asparaginyl endopeptidase). Our study populations were chosen from two villages in an endemic area close to Alexandria. Using fusion proteins MS2-Sm31 and MS2-Sm32 as antigens, 70% and 78.9%, respectively, of patient sera from 134 parasitologically confirmed cases reacted positively. The percentage of seropositivity increased to 84.5% when parasite-derived proteins Sm31 and Sm32 were used. The serum levels of antibodies to these two proteins in recombinant or native forms do not correlate with intensity of infection and hence are detected even when egg counts are low, which makes proteins Sm31 and Sm32 useful antigens in the identification of S. mansoni infected cases, particularly in endemic areas in Egypt.
我们进行了一系列酶联免疫吸附测定（ELISA），以评估两种曼氏血吸虫蛋白Sm31（半胱氨酸蛋白酶，组织蛋白酶B）和Sm32（天冬酰胺基内肽酶）的诊断意义。我们的研究对象选自亚历山大港附近一个流行地区的两个村庄。使用融合蛋白MS2-Sm31和MS2-Sm32作为抗原，来自134例经寄生虫学确诊病例的患者血清中，分别有70%和78.9%呈阳性反应。当使用寄生虫来源的蛋白Sm31和Sm32时，血清阳性率增至84.5%。重组形式或天然形式的这两种蛋白的抗体血清水平与感染强度无关，因此即使虫卵计数很低时也能检测到，这使得蛋白Sm31和Sm32成为曼氏血吸虫感染病例识别中的有用抗原，尤其是在埃及的流行地区。
