Livesey G, Wilson P D, Roe M A, Faulks R M, Oram L M, Brown J C, Eagles J, Greenwood R H, Kennedy H
Department of Nutrition, Diet, and Health, Institute of Food Research, Norwich NR4 7UA, United Kingdom.
Am J Physiol. 1998 Oct;275(4):E709-16. doi: 10.1152/ajpendo.1998.275.4.E709.
Estimates of the spanchnic retention and appearance in the systemic circulation of orally administered glucose vary among laboratories even after recently identified sources of error have been accounted for [Livesey, G., P. D. G. Wilson, J. R. Dainty, J. C. Brown, R. M. Faulks, M. A. Roe, T. A. Newman, J. Eagles, F. A. Mellon, and R. Greenwood. Am. J. Physiol. 275 (Endocrinol. Metab. 38): E717-E728, 1998]. We questioned whether, in healthy humans, D-glucose delivered intraluminally to the midjejunum appeared systemically as extensively as that delivered intraduodenally. Subjects were infused over a period of 90 min with 50 g of glucose in 1 liter of isotonic saline (incorporating 0.5 g D-[13C6]glucose) per 70 kg of body weight. Infusions were via enteral tubes terminating approximately 15 and 100 cm postpylorus. The systemic appearance of glucose was monitored by means of a primed-continuous intravenous infusion of D-[6,6-2H2]glucose. Whereas 98 +/- 2% (n = 7) of the duodenally infused glucose appeared in the systemic circulation, only 35 +/- 9% (n = 7) of midjejunally infused glucose did so, implying that 65 +/- 9% was retained in the splanchnic bed. Either glucose was less efficiently absorbed at the midintestinal site or hepatic glucose sequestration was increased 10-fold, or both. The proximal intestine plays a key role in the delivery of glucose to the systemic circulation, and the distal intestine potentially delivers more glucose to the liver.
即使在考虑了最近发现的误差来源之后，各实验室对口服葡萄糖在内脏的潴留量以及其在体循环中的出现情况的估计仍存在差异[利夫西，G.，P.D.G.威尔逊，J.R.丹蒂，J.C.布朗，R.M.福尔克斯，M.A.罗，T.A.纽曼，J.伊格尔斯，F.A.梅隆，以及R.格林伍德。《美国生理学杂志》275卷（内分泌与代谢38）：E717 - E728，1998年]。我们质疑，在健康人体中，经腔内输送至空肠中部的D - 葡萄糖在体循环中的出现程度是否与经十二指肠输送的葡萄糖一样广泛。以每70千克体重在1升等渗盐水中输注50克葡萄糖（含0.5克D - [13C6]葡萄糖）的方式，对受试者进行90分钟的输注。输注通过分别在幽门后约15厘米和100厘米处终止的肠内导管进行。通过对D - [6,6 - 2H2]葡萄糖进行首剂量连续静脉输注来监测葡萄糖在体循环中的出现情况。经十二指肠输注的葡萄糖有98±2%（n = 7）出现在体循环中，而经空肠中部输注的葡萄糖仅有35±9%（n = 7）出现，这意味着65±9%被潴留在内脏床。要么葡萄糖在肠中部的吸收效率较低，要么肝脏对葡萄糖的摄取增加了10倍，或者两者皆有。近端肠道在将葡萄糖输送至体循环中起关键作用，而远端肠道可能会将更多葡萄糖输送至肝脏。
