Villavedra M, Carol H, Nieto A
Cátedra de Inmunologia, Facultad de Química, Montevideo, Uruguay.
Rev Inst Med Trop Sao Paulo. 1998 Mar-Apr;40(2):77-84. doi: 10.1590/s0036-46651998000200003.
The recognition profile of the tissue cysts antigens by IgG antibodies was studied during acute and chronic human toxoplasmic infection. Thus the IgG response against Toxoplasma gondii was investigated by immunoblotting in two patients accidentally infected with the RH strain as well as in group of naturally infected patients at acute and chronic phase. There was an overall coincidence of molecular mass among antigens of tachyzoites and tissue cysts recognized by these sera, however, they appear not to be the same molecules. The response against tissue cysts starts early during acute infection, and the reactivity of antibodies is strong against a wide range of antigens. Six bands (between 82 and 151 kDa) were exclusively recognized by chronic phase sera but only the 132 kDa band was positive in more than 50% of the sera analysed. A mixture of these antigens could be used to discriminate between the two infection phases. The most important antigens recognized by the acute and the chronic phase sera were 4 clusters in the ranges 20-24 kDa, 34-39 kDa, 58-80 kDa and 105-130 kDa as well as two additional antigens of 18 and 29 kDa. Both accidentally infected patients and some of the naturally infected patients showed a weak specific response against tissue cyst antigens.
在人类急性和慢性弓形虫感染期间，研究了IgG抗体对组织包囊抗原的识别情况。因此，通过免疫印迹法对2例意外感染RH株的患者以及一组处于急性和慢性期的自然感染患者的弓形虫IgG反应进行了研究。这些血清识别的速殖子和组织包囊抗原的分子量总体上是一致的，然而，它们似乎不是相同的分子。针对组织包囊的反应在急性感染早期就开始了，并且抗体对多种抗原的反应性很强。6条带（82至151 kDa之间）仅被慢性期血清识别，但在分析的血清中，只有132 kDa的条带在超过50%的血清中呈阳性。这些抗原的混合物可用于区分两个感染阶段。急性期和慢性期血清识别的最重要抗原是4个簇，范围在20 - 24 kDa、34 - 39 kDa、58 - 80 kDa和105 - 130 kDa，以及另外两个18 kDa和29 kDa的抗原。意外感染的患者和一些自然感染的患者对组织包囊抗原均表现出较弱的特异性反应。
