H4IIE大鼠肝癌细胞和原代肝细胞中Mi-2自身抗原mRNA的等渗调节。

Anisoosmotic regulation of the Mi-2 autoantigen mRNA in H4IIE rat hepatoma cells and primary hepatocytes.

作者信息

Hammermann R, Warskulat U, Häussinger D

机构信息

Medizinische Universitätsklinik der Heinrich-Heine-Universität Düsseldorf, Klinik für Gastroenterologie, Hepatologie und Infektiologie, Germany.

出版信息

FEBS Lett. 1998 Sep 11;435(1):21-4. doi: 10.1016/s0014-5793(98)01030-8.

DOI:10.1016/s0014-5793(98)01030-8

PMID:9755851

Abstract

Using the differential display polymerase chain reaction (DDRT-PCR) a 169 bp cDNA product, which is 88.8% homologous to the human Mi-2beta autoantigen, was identified in H4IIE rat hepatoma cells. At protein level 100% homology was found. The Mi-2 mRNA was downregulated after hypoosmotic exposure and upregulated after hyperosmotic exposure in H4IIE cells and rat hepatocytes. The human Mi-2 is an autoantigen in dermatomyositis and is a member of the SNF/RAD 54 helicase family. Accordingly, Mi-2 may not only be a target of osmosignalling but could also be involved in the osmosignalling pathway towards gene expression in H4IIE and liver parenchymal cells.

摘要

运用差异显示聚合酶链反应（DDRT-PCR），在H4IIE大鼠肝癌细胞中鉴定出一个169 bp的cDNA产物，它与人Mi-2β自身抗原的同源性为88.8%。在蛋白质水平上发现同源性为100%。在H4IIE细胞和大鼠肝细胞中，低渗暴露后Mi-2 mRNA表达下调，高渗暴露后上调。人Mi-2是皮肌炎中的一种自身抗原，是SNF/RAD 54解旋酶家族的成员。因此，Mi-2不仅可能是渗透信号的靶点，还可能参与H4IIE细胞和肝实质细胞中通向基因表达的渗透信号通路。