McNew J A, Coe J G, Søgaard M, Zemelman B V, Wimmer C, Hong W, Söllner T H
Cellular Biochemistry and Biophysics Program, Memorial Sloan-Kettering Cancer Center, New York 10021, USA.
FEBS Lett. 1998 Sep 11;435(1):89-95. doi: 10.1016/s0014-5793(98)01044-8.
Specific transport between secretory compartments requires that vesicular carriers contain targeting proteins known as SNAREs. Ten v-SNAREs have been identified in the genome of the yeast Saccharomyces cerevisiae by sequence analysis. We report here the characterization of Gos1p, a v-SNARE localized to the Golgi compartment and likely homolog of the mammalian protein GOS-28/GS28. Gos1p is a type II membrane protein with characteristic SNARE sequence hallmarks and is functionally a SNARE protein. Gos1p was originally identified as a 28 kDa protein in an immunoprecipitate of the cis-Golgi t-SNARE Sed5p. This interaction between Sed5p and Gos1p is direct as demonstrated by in vitro binding with recombinant proteins. Deletion of GOS1 results in viable haploids with modest growth and secretory defects. Close examination of the secretory phenotype of GOS1-disrupted cells suggests that Gos1p may play a role in multiple transport steps, specifically ER-Golgi and/or intra-Golgi transport.
分泌区室之间的特异性转运要求囊泡载体含有被称为SNARE的靶向蛋白。通过序列分析，在酿酒酵母基因组中已鉴定出10种v-SNARE。我们在此报告Gos1p的特性，它是一种定位于高尔基体区室的v-SNARE，可能是哺乳动物蛋白GOS-28/GS28的同源物。Gos1p是一种II型膜蛋白，具有特征性的SNARE序列特征，在功能上是一种SNARE蛋白。Gos1p最初在顺式高尔基体t-SNARE Sed5p的免疫沉淀物中被鉴定为一种28 kDa的蛋白。Sed5p与Gos1p之间的这种相互作用是直接的，这已通过与重组蛋白的体外结合得到证实。GOS1的缺失导致具有适度生长和分泌缺陷的可存活单倍体。对GOS1缺失细胞的分泌表型进行仔细检查表明，Gos1p可能在多个转运步骤中发挥作用，特别是在内质网-高尔基体和/或高尔基体内部转运中。
