Diaz R, Kremer K, de Haas P E, Gomez R I, Marrero A, Valdivia J A, van Embden J D, van Soolingen D
National Reference Laboratory on Tuberculosis and Mycobacteria, Institute Pedro Kouri, Havana, Cuba.
Int J Tuberc Lung Dis. 1998 Sep;2(9):743-50.
Molecular typing has become an important tool for examining the extent of active transmission of tuberculosis.
To examine transmission of tuberculosis in Cuba using IS6110 restriction fragment length polymorphism (RFLP) typing and to evaluate the utility of spoligotyping.
One hundred and sixty Mycobacterium tuberculosis strains isolated over a one year period in Cuba were subjected to RFLP and spoligotyping.
Forty-eight percent of the isolates were found in 19 clusters of strains with identical RFLP patterns. In general, cluster sizes were limited, except for two large institutional outbreaks. Age was strongly inversely correlated to clustering. Most streptomycin-resistant isolates were found in clusters. Fifteen spoligotype clusters comprised 78% of the isolates. Significantly different IS6110 RFLP types subdivided 11 spoligotype clusters, whereas none of the IS6110 clusters were subdivided by spoligotyping.
Considering the short study period, 48% clustering is high, indicating that recent transmission plays an important role in Cuba. Although resistance is still a minor problem, transmission of streptomycin-resistant strains occurs. The high polymorphism observed with IS6110 RFLP indicates that this marker is useful for future molecular epidemiological studies in Cuba. Spoligotyping appeared less suitable for population-based studies.
分子分型已成为检测结核病主动传播程度的重要工具。
利用IS6110限制性片段长度多态性（RFLP）分型检测古巴结核病的传播情况，并评估间隔寡核苷酸分型技术（spoligotyping）的实用性。
对古巴一年内分离出的160株结核分枝杆菌菌株进行RFLP和间隔寡核苷酸分型。
48%的分离株存在于19个具有相同RFLP模式的菌株簇中。一般来说，除了两起大型机构性暴发外，菌株簇规模有限。年龄与菌株簇形成呈强烈负相关。大多数耐链霉素分离株存在于菌株簇中。15个间隔寡核苷酸分型簇包含78%的分离株。显著不同的IS6110 RFLP类型细分了11个间隔寡核苷酸分型簇，而没有一个IS6110簇被间隔寡核苷酸分型细分。
考虑到研究周期较短，48%的聚类率较高，表明近期传播在古巴起着重要作用。虽然耐药性仍是一个较小的问题，但耐链霉素菌株仍有传播。IS6110 RFLP观察到的高多态性表明该标记物对古巴未来的分子流行病学研究有用。间隔寡核苷酸分型似乎不太适合基于人群的研究。
