Vaughan R W, Zurowska A, Moszkowska G, Kondeatis E, Clark A G
South Thames Tissue Typing, Guy's Hospital, London, UK.
Tissue Antigens. 1998 Aug;52(2):130-4. doi: 10.1111/j.1399-0039.1998.tb02276.x.
We have studied the HLA-DRB and -DQB1 alleles of 42 paediatric patients who have suffered from membranous nephropathy associated with a hepatitis B infection (HBVMN). These patients were all from the Gdansk area of Northern Poland and the disease was diagnosed by light and electron microscopy. The control population consisted of 55 healthy children, approximately age matched, from schools in Gdansk. In addition we have also analysed 40 patients chronically infected with hepatitis B, without any renal involvement, as hepatitis B disease controls. The HLA alleles were defined using PCR/SSP. As idiopathic membranous nephropathy and low responsiveness to hepatitis B vaccine have been found to be associated with DR3 in Caucasoids, our hypothesis was that the HBVMN patients would show an increase in DR3. Our results indicate that, although there is a small increase in the frequency of DRBl0301 in the HBVMN patients (16/42 38%) when compared to the healthy controls (15/55 31%), this does not approach significance. There is a significant increase in the frequency of DQB10303 in the HBVMN patients vs the healthy controls, after correction for the number of antigens detected (Pc)(13/42 vs 2/55, RR=11.6, P=0.0007, Pc=0.02). A similar increase in DQB1*0303 is seen in the HBVMN patients when compared to the hepatitis controls (13/42 vs 4/40) but this is only significant before correction (RR=4.3, P=0.04).
我们研究了42例患有与乙型肝炎感染相关的膜性肾病（HBVMN）的儿科患者的HLA - DRB和 - DQB1等位基因。这些患者均来自波兰北部的格但斯克地区，疾病通过光镜和电镜诊断。对照组由55名年龄大致匹配的格但斯克学校的健康儿童组成。此外，我们还分析了40例慢性乙型肝炎感染且无任何肾脏受累的患者，作为乙型肝炎疾病对照。使用PCR / SSP定义HLA等位基因。由于在白种人中发现特发性膜性肾病和对乙型肝炎疫苗的低反应性与DR3相关，我们的假设是HBVMN患者的DR3会增加。我们的结果表明，尽管与健康对照组（15/55，31%）相比，HBVMN患者中DRB10301的频率略有增加（16/42，38%），但这并不具有统计学意义。校正检测到的抗原数量后（Pc），HBVMN患者中DQB10303的频率与健康对照组相比有显著增加（13/42对2/55，RR = 11.6，P = 0.0007，Pc = 0.02）。与乙型肝炎疾病对照组相比，HBVMN患者中DQB1*0303也有类似增加（13/42对4/40），但仅在校正前具有统计学意义（RR = 4.3，P = 0.04）。
