Gemeiner P, Docolomanský P, Vikartovská A, Stefuca V
Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, SK-842 38 Bratislava, Slovak Republic.
Biotechnol Appl Biochem. 1998 Oct;28 ( Pt 2):155-61.
This paper demonstrates a positive influence of a special, stepwise technique of enzyme immobilization based on the biospecific adsorption of the glycoenzyme invertase on immobilized concanavalin A (Con A), subsequent adsorption of the free Con A on the immobilized invertase:Con A support and repeated adsorption of invertase on the support. A 3-fold repetition of the same procedure designed preliminarily as bioaffinity layering afforded up to a 10-fold increase in catalytic activity of the immobilized invertase. Reactive hydrogels based on bead cellulose and bead poly(glycidyl methacrylate) were used as immobilization supports for the preparation of these highly active preparations. The enhancement in catalytic activity of immobilized invertase preparations was demonstrated thermometrically, by flow microcalorimetry. Further attractive aspects for utilizing the signal amplification of biosensors with immobilized enzymes are discussed.
本文展示了一种特殊的逐步酶固定化技术的积极影响，该技术基于糖酶转化酶在固定化伴刀豆球蛋白A（Con A）上的生物特异性吸附，随后游离的Con A吸附在固定化转化酶：Con A载体上，以及转化酶在载体上的重复吸附。将最初设计为生物亲和分层的相同程序重复3次，可使固定化转化酶的催化活性提高多达10倍。基于珠状纤维素和珠状聚（甲基丙烯酸缩水甘油酯）的反应性水凝胶用作固定化载体，以制备这些高活性制剂。通过流动微量热法以热分析法证明了固定化转化酶制剂催化活性的增强。还讨论了利用固定化酶的生物传感器信号放大的其他吸引人的方面。
