Aoyama Y, Horiuchi T, Gotoh O, Noshiro M, Yoshida Y
Department of Bioengineering, Faculty of Engineering, Soka University, Hachioji, 192-0003, Japan.
J Biochem. 1998 Oct;124(4):694-6. doi: 10.1093/oxfordjournals.jbchem.a022167.
A CYP51-like gene (Z80226) of Mycobacterium tuberculosis was expressed in Escherichia coli. The product exhibited absorption spectra characteristic of P450. The expressed P450 formed a stoichiometric complex with ketoconazole, one of the specific ligands of CYP51. These findings indicate that the CYP51-like gene of M. tuberculosis actually encodes a P450 having active-site environments similar to those of CYP51, confirming the predicted orthologous nature of this gene to eukaryotic CYP51. Although eukaryotic CYP51s are membrane-binding proteins, the expressed product was accumulated only in the soluble fraction of the host cells.
结核分枝杆菌的一个细胞色素P450 51样基因（Z80226）在大肠杆菌中表达。该产物呈现出细胞色素P450特有的吸收光谱。所表达的细胞色素P450与酮康唑（细胞色素P450 51的特异性配体之一）形成化学计量复合物。这些发现表明，结核分枝杆菌的细胞色素P450 51样基因实际上编码一种具有与细胞色素P450 51相似活性位点环境的细胞色素P450，证实了该基因与真核细胞色素P450 51预测的直系同源性质。尽管真核细胞色素P450 51是膜结合蛋白，但所表达的产物仅积累在宿主细胞的可溶性部分中。
