小鼠犁鼻器受体基因的分离及其与特定G蛋白信使核糖核酸的共定位

Isolation of mouse vomeronasal receptor genes and their co-localization with specific G-protein messenger RNAs.

作者信息

Saito H, Mimmack M L, Keverne E B, Kishimoto J, Emson P C

机构信息

Sub-Department of Animal Behavior, Department of Zoology, University of Cambridge, Madingley, Cambridge CB3 8AA, UK.

出版信息

Brain Res Mol Brain Res. 1998 Oct 1;60(2):215-27. doi: 10.1016/s0169-328x(98)00183-1.

DOI:10.1016/s0169-328x(98)00183-1

PMID:9757043

Abstract

Four mouse vomeronasal receptors (mV1Rs) have been isolated by similarity to rat vomeronasal receptor (V1R) motifs. The four mV1Rs identified in this study are members of two distinct subfamilies. Specific in situ hybridization probes (ISH) derived from the 3' non-coding regions of the mV1R genes, were used to detect expression of a single receptor and probes from the homologous coding regions were used to detect expression of subfamily members. The ISH results showed that the mV1Rs expressing neurons were scattered in the middle/upper layer of the vomeronasal organ (VNO) sensory epithelium in serial VNO sections but were excluded from the deeper layers of the VNO sensory epithelium and these neurons were found to co-express the mRNA for the G-protein Galphai2, and were distinct from the deeper layers of the VNO sensory epithelium where the mRNA for Galphao positive neurons was located.

摘要

通过与大鼠犁鼻器受体（V1R）基序的相似性，已分离出四种小鼠犁鼻器受体（mV1R）。本研究中鉴定出的四种mV1R是两个不同亚家族的成员。源自mV1R基因3'非编码区的特异性原位杂交探针（ISH）用于检测单个受体的表达，而来自同源编码区的探针用于检测亚家族成员的表达。ISH结果表明，表达mV1R的神经元散布在犁鼻器（VNO）感觉上皮的中/上层连续VNO切片中，但被排除在VNO感觉上皮的较深层之外，并且发现这些神经元共表达G蛋白Galphai2的mRNA，与Galphao阳性神经元mRNA所在的VNO感觉上皮较深层不同。