Tamagawa K, Fukushima S, Kobori M, Shinmoto H, Tsushida T
Research and Development Center, Hakubaku Co., Ltd., Yamanashi, Japan.
Biosci Biotechnol Biochem. 1998 Aug;62(8):1483-7. doi: 10.1271/bbb.62.1483.
Polyphenol extract from barley bran (BPE) induced nitro blue tetrazolium (NBT) reducing activity and alpha-naphthyl butyrate esterase activity in HL60 human myeloid leukemia cells. Because BPE induced the biochemical markers of HL60 cell differentiation, we investigated the effects of proanthocyanidins isolated from BPE on the HL60 cell differentiation of HL60 cells. Prodelphinidin B-3, T1, T2, and T3 induced 26-40% NBT-positive cells and 22-32% alpha-naphthyl butyrate esterase-positive cells. Proanthocyanidins potentiated retinoic acid (all-trans-retinoic acid)-induced granulocytic and sodium butyrate-induced monocytic differentiation in HL60 cells.
大麦麸皮多酚提取物（BPE）可诱导HL60人髓系白血病细胞中的硝基蓝四氮唑（NBT）还原活性和α-萘丁酸酯酶活性。由于BPE可诱导HL60细胞分化的生化标志物，因此我们研究了从BPE中分离出的原花青素对HL60细胞HL60细胞分化的影响。原花青素B-3、T1、T2和T3可诱导26%-40%的NBT阳性细胞和22%-32%的α-萘丁酸酯酶阳性细胞。原花青素可增强维甲酸（全反式维甲酸）诱导的HL60细胞粒细胞分化以及丁酸钠诱导的HL60细胞单核细胞分化。
