Crider J Y, Griffin B W, Xu S X, Sharif N A
Alcon Laboratories (R2-43), Forth Worth, Texas 76134-2099, USA.
Prostaglandins Leukot Essent Fatty Acids. 1998 Jul;59(1):77-82. doi: 10.1016/s0952-3278(98)90055-2.
The aim of these studies was to compare the effects of several prostaglandin agonists on adenylyl cyclase activity in embryonic bovine tracheal (EBTr) cells, transformed human nonpigmented ciliary epithelial (NPE) cells and National Cancer Bank (NCB-20) cells. These cell types have been shown to express DP, EP2 and IP prostaglandin (PG) receptors, respectively. Cyclic AMP (cAMP) generation was measured by manual and semi-automated radioimmunoassay (RIA) techniques. ZK118182 (EC50 = 10-27 nM), PGE2 (EC50 = 21-27 nM) and PGI2 (EC50 = 3.5-4 nM) had the highest potency at the DP, EP2 and IP receptors, respectively. A plot of potency (EC50) values generated with both techniques showed a high degree of correlation for all three receptors. These studies provide further characterization of prostanoid receptor functional responses in three cell types and demonstrate the advantages of a semi-automated RIA method for the analysis of the second messenger cAMP.
这些研究的目的是比较几种前列腺素激动剂对胚胎牛气管（EBTr）细胞、转化的人无色素睫状上皮（NPE）细胞和国家癌症库（NCB - 20）细胞中腺苷酸环化酶活性的影响。已证明这些细胞类型分别表达DP、EP2和IP前列腺素（PG）受体。通过手动和半自动放射免疫测定（RIA）技术测量环磷酸腺苷（cAMP）的生成。ZK118182（EC50 = 10 - 27 nM）、前列腺素E2（PGE2，EC50 = 21 - 27 nM）和前列腺素I2（PGI2，EC50 = 3.5 - 4 nM）分别在DP、EP2和IP受体上具有最高活性。用这两种技术生成的效价（EC50）值图显示，所有三种受体都具有高度相关性。这些研究进一步描述了三种细胞类型中前列腺素受体的功能反应，并证明了半自动RIA方法在分析第二信使cAMP方面的优势。
